Okumoto M, Nishikawa R, Iwai M, Iwai Y, Takamori Y, Niwa O, Yokoro K
Department of Medical Biology and Hygiene, Osaka Prefectural Radiation Research Institute, Sakai, Japan.
Radiat Res. 1990 Mar;121(3):267-73.
Southern blot analysis revealed no difference between the DNA from radiation-induced thymic lymphomas and DNA from normal NFS mice. The probes used in the Southern blot analyses used a murine leukemia virus (MuLV) env DNA probe (pXenv), which specifically hybridizes with xenotropic and recombinant viral env genes, and mouse mammary tumor virus (MMTV) DNA probes (MMTV gag-pol, MMTV env, and MMTV LTR). This suggests that radiation leukemogenesis was not associated with gross alteration of the organization of these retroviral genomes. In DNA from radiation-induced thymic lymphoma, there was no indication of gross rearrangement in the common integration site of MuLV, pim-1, or in the common integration sites of MMTV, int-1 and int-2. Dot blot analysis of RNA from radiation-induced thymic lymphomas and normal thymuses demonstrated that there was no substantial difference between them in the expression of retroviral sequences, pim-1, pvt-1, int-1, or int-2, although transcripts that could be hybridized to the retroviral sequences were slightly elevated in some radiation-induced thymic lymphomas. These results show that radiation leukemogenesis does not appear to involve the activation of endogenous type-C and type-B retroviruses.
Southern印迹分析显示，辐射诱导的胸腺淋巴瘤的DNA与正常NFS小鼠的DNA之间没有差异。Southern印迹分析中使用的探针包括鼠白血病病毒（MuLV）env DNA探针（pXenv），它能与嗜异源性和重组病毒env基因特异性杂交，以及小鼠乳腺肿瘤病毒（MMTV）DNA探针（MMTV gag-pol、MMTV env和MMTV LTR）。这表明辐射诱导白血病发生与这些逆转录病毒基因组组织的总体改变无关。在辐射诱导的胸腺淋巴瘤的DNA中，没有迹象表明MuLV、pim-1的共同整合位点或MMTV、int-1和int-2的共同整合位点发生了总体重排。对辐射诱导的胸腺淋巴瘤和正常胸腺的RNA进行斑点印迹分析表明，它们在逆转录病毒序列、pim-1、pvt-1、int-1或int-2的表达上没有实质性差异，尽管在一些辐射诱导的胸腺淋巴瘤中，可与逆转录病毒序列杂交的转录本略有升高。这些结果表明，辐射诱导白血病发生似乎不涉及内源性C型和B型逆转录病毒的激活。
