Astier-Gin T, Legrand E, Baylac-Kalabokias H, Guillemain B
INSERM U.117, Bordeaux, France.
Leukemia. 1990 Apr;4(4):307-11.
The role of B ecotropic recombinant retroviruses in the emergence and the progression of radio-induced thymic lymphomas was evaluated by analyzing the cell populations present in nine primary and in in vivo propagated tumors. For this, tumor DNAs were analyzed by the Southern method using probes specific for newly acquired proviral sequences, T-cell receptor beta-chain, and immunoglobulin heavy chain genes. Our results show that primary radio-induced tumors are composed of several tumoral cell clones but do not support that malignant cell transformation and proliferation are conferred, solely, by the newly acquired ecotropic recombinant retroviral sequences.
通过分析九个原发性肿瘤和体内传代肿瘤中的细胞群体，评估亲嗜性重组逆转录病毒在放射性诱导胸腺淋巴瘤的发生和发展中的作用。为此，使用针对新获得的前病毒序列、T细胞受体β链和免疫球蛋白重链基因的探针，通过Southern方法分析肿瘤DNA。我们的结果表明，原发性放射性诱导肿瘤由多个肿瘤细胞克隆组成，但并不支持恶性细胞转化和增殖仅由新获得的亲嗜性重组逆转录病毒序列赋予。
