Wubet Tesfaye, Weiss Michael, Kottke Ingrid, Teketay Demel, Oberwinkler Franz
Universität Tübingen, Spezielle Botanik und Mykologie, Auf der Morgenstelle 1, D-72076 Tübingen, Germany.
Mycol Res. 2006 Sep;110(Pt 9):1059-69. doi: 10.1016/j.mycres.2006.04.005. Epub 2006 Aug 10.
The endangered indigenous tree species Juniperus procera, commonly known as African Pencil Cedar, is an important component of the dry Afromontane vegetation of Ethiopia and was shown to be AM in earlier studies. Here we describe the composition of AM fungi in colonized roots of J. procera from two dry Afromontane forests of Ethiopia. The nuSSU rDNA gene was amplified from colonized roots, cloned and sequenced using AM fungal specific primers that were partly developed for this study. Molecular phylogenetic analysis revealed that all the glomeralean sequences obtained belonged exclusively to the genus Glomus (Glomeraceae). Seven distinct Glomus sequence types were identified that all are new to science. The composition of the AM fungal communities between the sampled trees, and between the two study sites in general, differed significantly. Isolation and utilization of the indigenous AM fungal taxa from the respective sites might be required for successful enrichment plantation of this threatened Juniperus species.
濒危本土树种刺柏（Juniperus procera），通常被称为非洲铅笔柏，是埃塞俄比亚干旱山地植被的重要组成部分，并且在早期研究中已被证明为丛枝菌根（AM）植物。在此，我们描述了来自埃塞俄比亚两个干旱山地森林的刺柏被侵染根系中丛枝菌根真菌的组成。使用部分为本研究开发的丛枝菌根真菌特异性引物，从被侵染的根系中扩增、克隆并测序了核小亚基核糖体DNA（nuSSU rDNA）基因。分子系统发育分析表明，所有获得的球囊霉目序列均仅属于球囊霉属（Glomeraceae）。鉴定出了7种不同的球囊霉序列类型，所有这些类型对科学界来说都是新的。采样树木之间以及两个研究地点之间的丛枝菌根真菌群落组成存在显著差异。对于成功培育这种受威胁的刺柏树种，可能需要从各自地点分离和利用本土丛枝菌根真菌类群。
