Molina T C, Brown H D, Irbe R M, Pierson D L
Biomedical Operations and Research Branch, NASA-Johnson Space Center, Houston, Texas 77058.
Appl Environ Microbiol. 1990 Mar;56(3):601-6. doi: 10.1128/aem.56.3.601-606.1990.
A self-contained, portable Gram staining apparatus (GSA) has been developed for use in the microgravity environment on board the Space Station Freedom. Accuracy and reproducibility of this apparatus compared with the conventional Gram staining method were evaluated by using gram-negative and gram-positive controls and different species of bacteria grown in pure cultures. A subsequent study was designed to assess the performance of the GSA with actual specimens. A set of 60 human and environmental specimens was evaluated with the GSA and the conventional Gram staining procedure. Data obtained from these studies indicated that the GSA will provide the Gram staining capability needed for the microgravity environment of space.
一种独立的便携式革兰氏染色仪（GSA）已被开发出来，用于在“自由号”空间站的微重力环境中使用。通过使用革兰氏阴性和革兰氏阳性对照以及在纯培养物中生长的不同种类细菌，评估了该仪器与传统革兰氏染色方法相比的准确性和可重复性。随后设计了一项研究来评估GSA对实际标本的性能。用GSA和传统革兰氏染色程序对一组60份人类和环境标本进行了评估。从这些研究中获得的数据表明，GSA将提供太空微重力环境所需的革兰氏染色能力。
