Racz T, Sacks P, Van N T, Taylor D L, Young G, Bugis S, Savage H E, Schantz S P
Department of Head and Neck Surgery, National Institute of Oncology, Budapest, Hungary.
Arch Otolaryngol Head Neck Surg. 1990 Apr;116(4):440-6. doi: 10.1001/archotol.1990.01870040062015.
A flow cytometric assay was used to detect the lytic and binding capacities of both fresh peripheral blood lymphocytes and purified Leu-19+ natural killer cells against head and neck cancer cell lines. Results demonstrated that natural killer cell-mediated cytotoxicity and effector-target conjugate formation evaluated by flow cytometry was significantly correlated with the standard chromium 51 release assay and the single-cell microscopic assay, respectively. The sorted Leu-19+ natural killer cells demonstrated higher lytic capacity with a corresponding higher binding rate compared with the unsorted peripheral blood lymphocytes and sorted Leu-19- cells. Flow cytometric analysis of natural killer cell activity (a rapid, simple, and quantifiable procedure) is an alternative to the standard chromium 51 release assay.
采用流式细胞术检测新鲜外周血淋巴细胞和纯化的Leu-19+自然杀伤细胞对头颈癌细胞系的杀伤和结合能力。结果表明，通过流式细胞术评估的自然杀伤细胞介导的细胞毒性和效应细胞-靶细胞共轭形成分别与标准的铬51释放试验和单细胞显微镜试验显著相关。与未分选的外周血淋巴细胞和分选的Leu-19-细胞相比，分选的Leu-19+自然杀伤细胞表现出更高的杀伤能力和相应更高的结合率。自然杀伤细胞活性的流式细胞术分析（一种快速、简单且可量化的方法）是标准铬51释放试验的替代方法。
