Afify Z A, Abdel-Mageed A, Findley H W, Ragab A H
Division of Pediatric Hematology/Oncology, Emory University, Atlanta, Georgia.
Cancer. 1990 Aug 1;66(3):469-73. doi: 10.1002/1097-0142(19900801)66:3<469::aid-cncr2820660312>3.0.co;2-e.
Several investigators have reported decreased natural killer cell activity of the peripheral blood from children with acute leukemia both at diagnosis and in relapse. This study was to determine whether this decreased natural killer activity is the result of a diminished number or defective function of natural killer cells or both. The percentage of Leu 19+ (NKH-1, CD56) and the lytic activity of a purified Leu 19+ population from the peripheral blood of children with acute lymphocytic leukemia as well as from healthy children were determined. This cell population mediates most natural killer and natural killer-like activity in the peripheral blood. Twenty-three children with acute lymphocytic leukemia (16 at diagnosis, seven in relapse) as well as six control children were studied. The percentage of Leu 19+ cells was significantly lower in the blood of children either at initial diagnosis (median, 3%; P less than 0.005) or in relapse (median, 1%; P less than 0.01) than that for the control children (median, 12.5%). The cytotoxicity of peripheral blood mononuclear cells (PBMC) against K562 in 3-hour 51Cr release assay was significantly lower for patients either at diagnosis (median, 8.5 lytic units[LU]/10(7) cells; P less than 0.005) or in relapse (median, 3 LU/10(7) cells; P less than 0.005) than that for normal controls (median, 97 LU/10(7) cells). The lytic activity of a fluorescent activated cell sorter (FACS) purified Leu 19+ population was evaluated. The Leu 19+ cells were sorted from PBMC of 11 children with acute lymphocytic leukemia who had more than 2% Leu 19+ cells in their PBMC (nine at diagnosis and two in relapse). The lytic activity of sorted Leu 19+ cells was significantly (P less than 0.04) lower for patients at initial diagnosis (median, 45 LU/10(7) cells) than that for normal control children (median, 317 LU/10(7) cells). One of the two children studied at relapse had low Leu 19+ lytic activity. The authors concluded that (1) The percentage of Leu 19+ cells in the peripheral blood of the majority of children with acute lymphocytic leukemia either at diagnosis or in relapse is below normal; (2) The lytic activity of PBMC from the majority of children with acute lymphocytic leukemia is reduced both at diagnosis and in relapse; and (3) The lytic activity of Leu 19+ purified PBMC is reduced in the majority of children with acute lymphocytic leukemia. These findings confirm the defective natural killer cell profile in children with acute lymphocytic leukemia both at the time of diagnosis and in relapse.
几位研究者报告称，急性白血病患儿在诊断时和复发时外周血自然杀伤细胞活性均降低。本研究旨在确定这种自然杀伤活性降低是自然杀伤细胞数量减少还是功能缺陷或两者兼而有之的结果。测定了急性淋巴细胞白血病患儿以及健康儿童外周血中Leu 19+（NKH-1，CD56）的百分比和纯化的Leu 19+细胞群的溶解活性。该细胞群介导外周血中的大多数自然杀伤和自然杀伤样活性。对23例急性淋巴细胞白血病患儿（16例诊断时，7例复发时）以及6例对照儿童进行了研究。无论是初诊时（中位数为3%；P<0.005）还是复发时（中位数为1%；P<0.01），患儿血液中Leu 19+细胞的百分比均显著低于对照儿童（中位数为12.5%）。在3小时51Cr释放试验中，急性淋巴细胞白血病患者外周血单个核细胞（PBMC）对K562的细胞毒性在诊断时（中位数为8.5溶细胞单位[LU]/10(7)细胞；P<0.005）或复发时（中位数为3 LU/10(7)细胞；P<0.005）均显著低于正常对照（中位数为97 LU/10(7)细胞）。评估了荧光激活细胞分选仪（FACS）纯化的Leu 19+细胞群的溶解活性。从11例PBMC中Leu 19+细胞超过2%的急性淋巴细胞白血病患儿（9例诊断时，2例复发时）的PBMC中分离出Leu 19+细胞。初诊时患者分选的Leu 19+细胞的溶解活性（中位数为45 LU/10(7)细胞）显著低于正常对照儿童（中位数为317 LU/10(7)细胞）（P<0.04）。研究的2例复发患儿中有1例Leu 19+溶解活性较低。作者得出结论：（1）大多数急性淋巴细胞白血病患儿在诊断时或复发时外周血中Leu 19+细胞的百分比低于正常水平；（2）大多数急性淋巴细胞白血病患儿的PBMC在诊断时和复发时溶解活性均降低；（3）大多数急性淋巴细胞白血病患儿纯化的Leu 19+PBMC的溶解活性降低。这些发现证实了急性淋巴细胞白血病患儿在诊断时和复发时自然杀伤细胞谱存在缺陷。
