Heijink Andras, Yaszemski Michael J, Patel Robin, Rouse Mark S, Lewallen David G, Hanssen Arlen D
Department of Orthopedic Surgery, Mayo Clinic College of Medicine, Rochester, MN, USA.
Clin Orthop Relat Res. 2006 Oct;451:29-33. doi: 10.1097/01.blo.0000229319.45416.81.
Biodegradable local antibiotic delivery systems have gained interest for prophylaxis and treatment of musculoskeletal infections. We studied the biodegradable materials Osteo- Set, DBX and Collagraft for local delivery of vancomycin and gentamicin in vitro. We determined the antimicrobial activity of vancomycin and gentamicin after mixing with each biodegradable material and determined the release of each antimicrobial from each material in an intermittent flow chamber. Antimicrobial activity was expressed as percent of antimicrobial loaded into each sample that was detected; antimicrobial release was expressed as concentration (microg/mL) after timed intervals of chamber flow, peak concentration, area under the curve and percent antimicrobial recovered. Activity of vancomycin after mixing with Osteo- Set, DBX and Collagraft was > 73%. Activity of gentamicin after mixing with DBX was 100%; after mixing with OsteoSet and Collagraft it was reduced to < 61%. AUC0-48hrs of vancomycin was 469, 426 and 432 microg x hr/mL, and the AUC0-48hrs of gentamicin was 368, 306 and 301 microg x hr/mL after release from OsteoSet, DBX, and Collagraft, respectively. Recovered percentages of vancomycin were 39%, 11% and 25%, and recovered percentages of gentamicin were 39%, 9% and 23% after release from OsteoSet, DBX, and Collagraft, respectively. OsteoSet, DBX and Collagraft may be suitable for local delivery of vancomycin and gentamicin.
可生物降解的局部抗生素递送系统已引起人们对肌肉骨骼感染预防和治疗的关注。我们研究了可生物降解材料Osteo-Set、DBX和Collagraft在体外局部递送万古霉素和庆大霉素的情况。我们在将万古霉素和庆大霉素与每种可生物降解材料混合后测定其抗菌活性，并在间歇流动室中测定每种抗菌药物从每种材料中的释放情况。抗菌活性以检测到的加载到每个样品中的抗菌药物百分比表示；抗菌药物释放以室流动定时间隔后的浓度（微克/毫升）、峰值浓度、曲线下面积和回收的抗菌药物百分比表示。万古霉素与Osteo-Set、DBX和Collagraft混合后的活性>73%。庆大霉素与DBX混合后的活性为100%；与OsteoSet和Collagraft混合后活性降至<61%。万古霉素从OsteoSet、DBX和Collagraft释放后的AUC0-48hrs分别为469、426和432微克×小时/毫升，庆大霉素的AUC0-48hrs分别为368、306和301微克×小时/毫升。万古霉素从OsteoSet、DBX和Collagraft释放后的回收百分比分别为39%、11%和25%，庆大霉素的回收百分比分别为39%、9%和23%。OsteoSet、DBX和Collagraft可能适用于局部递送万古霉素和庆大霉素。
