Houck K A, Zarnegar R, Muga S J, Michalopoulos G K
Department of Pathology, Duke University Medical Center, Durham, North Carolina.
J Cell Physiol. 1990 Apr;143(1):129-32. doi: 10.1002/jcp.1041430117.
Acidic fibroblast growth factor (aFGF) stimulated DNA synthesis in primary rat hepatocyte cultures in a dose-dependent manner with maximal effect at 10-50 ng ml-1. This activity was dependent on the presence of heparin at a concentration of 10-50 micrograms.ml-1. Insulin interacted synergistically with aFGF, as it did with epidermal growth factor (EGF). The response to aFGF was only 50% that found with EGF. The disparity was not due to different kinetics of DNA synthesis, since the peak response for both growth factors occurred at 36-72 hr after plating of the hepatocytes. The potential relevance of this novel hepatocyte mitogen to normal and pathological liver growth is discussed.
酸性成纤维细胞生长因子（aFGF）以剂量依赖方式刺激原代大鼠肝细胞培养物中的DNA合成，在10 - 50 ng/ml时具有最大效应。该活性依赖于浓度为10 - 50 μg/ml的肝素的存在。胰岛素与aFGF协同作用，就像它与表皮生长因子（EGF）协同作用一样。对aFGF的反应仅为对EGF反应的50%。这种差异并非由于DNA合成的动力学不同，因为两种生长因子的峰值反应均出现在肝细胞接种后36 - 72小时。讨论了这种新型肝细胞有丝分裂原与正常和病理性肝脏生长的潜在相关性。
