Itoh T, Suzuki M, Mitsui Y
Cell Biology Laboratory, National Institute of Bioscience and Human-Technology, Ibaraki, Japan.
Biochem Biophys Res Commun. 1993 May 14;192(3):1011-5. doi: 10.1006/bbrc.1993.1517.
In a series of our studies on identification of novel mitogens for hepatocytes, keratinocyte growth factor (KGF, FGF-7) is found to have a stimulatory effect on DNA synthesis of adult rat hepatocytes in primary culture. Stimulation of DNA synthesis with KGF was seen to be dose dependent with its maximum effect at 15-50ng/ml. Studies on nuclear labeling with BrdU confirmed the mitogenic effect of KGF on rat hepatocytes. The mitogenic effect of KGF was greater than those of EGF, FGF-1 and comparable to that of HGF. Further, it exhibited additive mitogenic effect when present along with the optimum concentration of HGF, EGF or FGF-1. Thus, it is concluded that KGF is a potent mitogen for rat hepatocytes in vitro.
在我们一系列关于鉴定肝细胞新型促分裂原的研究中，发现角质形成细胞生长因子（KGF，FGF - 7）对原代培养的成年大鼠肝细胞的DNA合成具有刺激作用。观察到用KGF刺激DNA合成呈剂量依赖性，在15 - 50ng/ml时具有最大效应。用BrdU进行核标记的研究证实了KGF对大鼠肝细胞的促有丝分裂作用。KGF的促有丝分裂作用大于表皮生长因子（EGF）、成纤维细胞生长因子 - 1（FGF - 1），与肝细胞生长因子（HGF）相当。此外，当与最佳浓度的HGF、EGF或FGF - 1同时存在时，它表现出相加的促有丝分裂作用。因此，得出结论：KGF在体外是大鼠肝细胞的一种强效促分裂原。
