Theodorou I D, Boumsell L, Calvo C F, Gouy H, Beral H M, Debre P
Laboratoire d'Immunologie Cellulaire et Tissulaire, URA CNRS 0186, Centre Hospitalier Pitié Salpétrière, Paris, France.
J Immunol. 1990 Apr 1;144(7):2518-23.
The effect of a battery of CD1 mAb on intracellular free Ca2+ concentration and IL-2 production has been examined on different T cell lines in this study. Both 0249F and NU-T2 two CD1b specific mAb tested, induced a rapid increase in the intracellular Ca2+ concentration on HPBALL T cells whereas only one (L161) among three different CD1c mAb (L161, 10C3, and M241) produced a similar effect. In contrast the addition of four different CD1a mAb directed against two different epitopic groups of this molecule were uneffective in modifying the intracellular Ca2+. Both L161 and 0249F also induced a comparable increase in the intracellular Ca2+ concentration on MOLT 4 and JURKAT, two other T cell lines of similar phenotype. The effect of L161 mAb on the IL-2 production of the IL-2 producing T cell line JURKAT was also examined. The association of the latter with PMA strongly induced the production of IL-2 on this cell model while either L161 or PMA alone had no effect. Although the natural ligand and the function of CD1 molecules are still unknown, the accumulation of these data strongly suggest that CD1b and CD1c might represent two activatory pathways for immature T cells operating before the classical CD2 and CD3 activation pathways.
本研究检测了一组CD1单克隆抗体（mAb）对不同T细胞系细胞内游离钙离子（Ca2+）浓度及白细胞介素-2（IL-2）产生的影响。所检测的两种CD1b特异性单克隆抗体0249F和NU-T2，均可使HPBALL T细胞内Ca2+浓度迅速升高，而在三种不同的CD1c单克隆抗体（L161、10C3和M241）中，只有一种（L161）产生了类似效应。相反，针对该分子两个不同表位组的四种不同CD1a单克隆抗体，在改变细胞内Ca2+浓度方面均无作用。L161和0249F在另外两种表型相似的T细胞系MOLT 4和JURKAT中，也可使细胞内Ca2+浓度产生类似升高。还检测了L161单克隆抗体对产生IL-2的T细胞系JURKAT的IL-2产生的影响。在该细胞模型中，L161与佛波酯（PMA）联合使用可强烈诱导IL-2产生，而单独使用L161或PMA均无作用。尽管CD1分子的天然配体和功能仍不清楚，但这些数据的积累强烈提示，CD1b和CD1c可能代表未成熟T细胞在经典CD2和CD3激活途径之前起作用的两种激活途径。
