Moon-van der Staay Seung Yeo, Tzeneva Vesela A, van der Staay Georg W M, de Vos Willem M, Smidt Hauke, Hackstein Johannes H P
Department of Evolutionary Microbiology, Faculty of Science, Radboud University Nijmegen, Nijmegen, The Netherlands.
FEMS Microbiol Ecol. 2006 Sep;57(3):420-8. doi: 10.1111/j.1574-6941.2006.00130.x.
The eukaryotic biodiversity in historical air-dried samples of Dutch agricultural soil has been assessed by random sequencing of an 18S rRNA gene library and by denaturing gradient gel electrophoresis. Representatives of nearly all taxa of eukaryotic soil microbes could be identified, demonstrating that it is possible to study eukaryotic microbiota in samples from soil archives that have been stored for more than 30 years at room temperature. In a pilot study, 41 sequences were retrieved that could be assigned to fungi and a variety of aerobic and anaerobic protists such as cercozoans, ciliates, xanthophytes (stramenopiles), heteroloboseans, and amoebozoans. A PCR-denaturing gradient gel electrophoresis analysis of samples collected between 1950 and 1975 revealed significant changes in the composition of the eukaryotic microbiota.
通过对18S rRNA基因文库进行随机测序以及变性梯度凝胶电泳，对荷兰农业土壤的历史风干样本中的真核生物多样性进行了评估。几乎所有真核土壤微生物类群的代表都能被鉴定出来，这表明研究在室温下储存了30多年的土壤档案样本中的真核微生物群是可行的。在一项初步研究中，检索到41个序列，这些序列可归类为真菌以及各种需氧和厌氧原生生物，如丝足虫、纤毛虫、黄藻（不等鞭毛类）、异叶足虫和变形虫。对1950年至1975年间采集的样本进行的PCR-变性梯度凝胶电泳分析显示，真核微生物群的组成发生了显著变化。
