Steven F S, Williams L A
Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Manchester, UK.
J Enzyme Inhib. 1990;3(4):317-22. doi: 10.3109/14756369009030381.
Leukaemia cells possess a latent form of a cell surface protease referred to as guanidinobenzoatase. Latency is due to complex formation between an inhibitor protein and the cell surface enzyme which is stable under acid conditions but is dissociated with formaldehyde treatment. The latent form of the cell surface protease has been used as a protecting mechanism during a preliminary step to stain all the nuclei of cells with haematoxylin. The enzyme-inhibitor complex was then dissociated and a combination of 9-amino acridine and propidium iodide employed to enable the fluorescent location of cells possessing active guanidinobenzoatase. We were thus able to visualise the nuclei by conventional light microscopy and simultaneously visualise the cell surface of leukaemia cells by fluorescent microscopy. This simple model system has provided technology applicable to the more complex analysis of neoplastic cells in cervical smears.
白血病细胞具有一种细胞表面蛋白酶的潜伏形式，称为胍基苯甲酰酶。潜伏是由于一种抑制蛋白与细胞表面酶之间形成复合物，该复合物在酸性条件下稳定，但经甲醛处理后会解离。细胞表面蛋白酶的潜伏形式在使用苏木精对所有细胞核进行染色的初步步骤中被用作一种保护机制。然后使酶 - 抑制剂复合物解离，并使用9 - 氨基吖啶和碘化丙啶的组合来实现对具有活性胍基苯甲酰酶的细胞进行荧光定位。因此，我们能够通过传统光学显微镜观察细胞核，同时通过荧光显微镜观察白血病细胞的细胞表面。这个简单的模型系统提供了适用于对宫颈涂片肿瘤细胞进行更复杂分析的技术。
