Steven F S, Griffin M M, Blakey D C, Talbot I C, Hanski C, Bell J
Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Manchester, U.K.
Anticancer Res. 1992 Nov-Dec;12(6B):2159-64.
Normal colonic epithelial cells possess a cell surface protease referred to as guanidinobenzoatase (GB) and a corresponding cytoplasmic protein inhibitor of GB. Colonic carcinoma cells possess two isoenzymic forms of GB, the normal and the carcinoma specific form, each of which is recognised by the corresponding inhibitors present in the cytoplasm of colonic carcinoma cells. An affinity-purified inhibitor preparation obtained from the cytoplasm of cultured colonic carcinoma cells inhibited these two colonic carcinoma isoenzymic forms of GB but not the GB associated with other forms of tumour. The data suggest that each cell type possessing isoenzymic forms of cell surface GB also possesses the corresponding cell-specific inhibitors of GB.
正常结肠上皮细胞拥有一种称为胍基苯甲酸酶（GB）的细胞表面蛋白酶以及相应的GB胞质蛋白抑制剂。结肠癌细胞拥有GB的两种同工酶形式，即正常形式和癌特异性形式，每一种都能被结肠癌细胞胞质中存在的相应抑制剂识别。从培养的结肠癌细胞胞质中获得的一种亲和纯化抑制剂制剂可抑制GB的这两种结肠癌细胞同工酶形式，但不抑制与其他肿瘤形式相关的GB。数据表明，每种拥有细胞表面GB同工酶形式的细胞类型也拥有相应的GB细胞特异性抑制剂。
