Dabholkar M, Tatake R J, Advani S, Gangal S G
Immunology Division, Tata Memorial Centre, Parel, Bombay, India.
Neoplasma. 1990;37(1):47-53.
In our earlier studies, the natural killer (NK) cell mediated cytotoxicity was found to be impaired in 55% of chronic myeloid leukemia (CML) patients in remission (low NK responders), while the rest exhibited normal range of cytotoxicity (normal NK responders). In the present investigations probable factors contributing to the impaired NK activity of low NK responder CML patients have been analyzed. Peripheral blood lymphocytes (PBL) from both low and normal NK responder CML patients possessed normal percentages of HNK-1+, CD3+ and CD8+ cells. The proportion of HNK-1+ cells and CD8+ cells in low density fractions (LDF) and high density fractions (HDF) respectively of Percoll separated PBL from low and normal NK responder CML patients and healthy donors was comparable. However, the NK activity of LDF cells of low NK responder CML patients was much lower. Also, HDF cells from low NK responder CML patients exhibited a regulatory effect on NK cytotoxicity of PBL from healthy donors. Therefore, it is likely that the presence of suppressor cells and perhaps an intrinsic inability of HNK-1+ cells may together contribute to the impaired NK cytotoxicity of low NK responder CML patients.
在我们早期的研究中，发现55%处于缓解期的慢性髓性白血病（CML）患者的自然杀伤（NK）细胞介导的细胞毒性受损（低NK反应者），而其余患者表现出正常范围的细胞毒性（正常NK反应者）。在本研究中，对导致低NK反应者CML患者NK活性受损的可能因素进行了分析。来自低NK反应者和正常NK反应者CML患者的外周血淋巴细胞（PBL）中HNK-1+、CD3+和CD8+细胞的百分比均正常。在低NK反应者和正常NK反应者CML患者以及健康供体经Percoll分离的PBL的低密度组分（LDF）和高密度组分（HDF）中，HNK-1+细胞和CD8+细胞的比例分别具有可比性。然而，低NK反应者CML患者LDF细胞的NK活性要低得多。此外，低NK反应者CML患者的HDF细胞对健康供体PBL的NK细胞毒性表现出调节作用。因此，抑制细胞的存在以及HNK-1+细胞可能存在的内在功能缺陷可能共同导致了低NK反应者CML患者NK细胞毒性的受损。
