Mori N, Oka K, Yoda Y, Abe T, Kojima M
Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan.
Am J Clin Pathol. 1990 Apr;93(4):495-501. doi: 10.1093/ajcp/93.4.495.
Twenty-eight cases with T-cell neoplasms (10 with T-cell acute lymphoblastic leukemia [T-ALL], 10 with T-lymphoblastic lymphoma, and 8 with peripheral T-cell lymphomas) and 2 cases with reactive lymph nodes were immunohistochemically stained with monoclonal antibodies beta F1, delta TCS1, and WT31; beta F1 antibody recognizes the beta-subunit of T-cell receptor (TCR), whereas delta TCS1 and WT31 recognize the delta- and alpha beta-subunits of TCR, respectively. Five cases with T-ALL, four with T-lymphoblastic lymphoma (T-LL), and seven with peripheral T-cell lymphomas were positive for beta F1. None showed positive reactivity for delta TCS1. One case with T-LL and four cases with peripheral T-cell lymphomas were positive for WT31. Of the nine cases positive for beta F1 among T-ALLs and T-LLs, six were also positive for CD1 (OKT6), whereas six of seven positive cases for CD1 were positive for beta F1. The authors therefore suggest that TCR beta is expressed in the immature T-cells just earlier than or around the same stage of differentiation as those expressing CD1. The authors' immuno-electron microscopy study revealed that positive reactivity for beta F1 was localized predominantly in the cytoplasm of the neoplastic cells in the cases with T-ALL, T-LL and peripheral T-cell lymphomas, and in the cytoplasm of the reactive T-cells. However, it was not localized on the surface membrane. In contrast, positive reactivity for WT31 was localized on the surface membrane of the neoplastic and reactive T-cells. Only half of the cases of peripheral T-cell lymphomas showed positive reactivity for WT31. The authors consider that it may not be a very useful antibody for the detection of TCR alpha beta on the T-cell neoplasms using frozen tissue sections.
对28例T细胞肿瘤患者（10例T细胞急性淋巴细胞白血病[T - ALL]、10例T淋巴母细胞淋巴瘤和8例外周T细胞淋巴瘤）以及2例反应性淋巴结进行免疫组织化学染色，使用单克隆抗体βF1、δTCS1和WT31；βF1抗体识别T细胞受体（TCR）的β亚基，而δTCS1和WT31分别识别TCR的δ亚基和αβ亚基。5例T - ALL、4例T淋巴母细胞淋巴瘤（T - LL）和7例外周T细胞淋巴瘤βF1呈阳性。δTCS1均无阳性反应。1例T - LL和4例外周T细胞淋巴瘤WT31呈阳性。在T - ALL和T - LL中βF1呈阳性的9例患者中，6例CD1（OKT6）也呈阳性，而7例CD1阳性病例中有6例βF1呈阳性。因此，作者认为TCRβ在未成熟T细胞中的表达早于或与表达CD1的细胞处于同一分化阶段。作者的免疫电镜研究显示，在T - ALL、T - LL和外周T细胞淋巴瘤病例中，βF1的阳性反应主要定位于肿瘤细胞的细胞质中，以及反应性T细胞的细胞质中。然而，它并不定位于表面膜。相比之下，WT31的阳性反应定位于肿瘤性和反应性T细胞的表面膜。仅一半的外周T细胞淋巴瘤病例WT31呈阳性反应。作者认为，对于使用冷冻组织切片检测T细胞肿瘤上的TCRαβ而言，它可能不是一种非常有用的抗体。
