通过银染法在聚丙烯酰胺凝胶上测定聚（ADP-核糖）链长度分布

Determination of poly(ADP-ribose) chain length distribution on polyacrylamide gels by silver staining.

作者信息

Hacham H, Ben-Ishai R

机构信息

Department of Biology, Technion-Israel Institute of Technology, Haifa.

出版信息

Anal Biochem. 1990 Jan;184(1):83-5. doi: 10.1016/0003-2697(90)90015-2.

DOI:10.1016/0003-2697(90)90015-2

PMID:1690959

Abstract

Silver staining and polyacrylamide gel electrophoresis were used to visualize chain length distribution of poly(ADP-ribose) enzymatically synthesized from NAD by rat liver nuclei. The method described has the advantage that synthesis does not require radioactive-labeled NAD, and microgram quantities (greater than 5 micrograms) of poly(ADP-ribose) can be resolved and visualized as discrete bands according to chain lengths which range from 8 to 60 residues. This method can be applied to estimate size distribution of poly(ADP-ribose) chains in cells or tissues.

摘要

采用银染和聚丙烯酰胺凝胶电泳来观察大鼠肝细胞核从NAD酶促合成的聚（ADP-核糖）的链长分布。所述方法的优点是合成不需要放射性标记的NAD，并且微克量（大于5微克）的聚（ADP-核糖）可以根据8至60个残基的链长解析并显示为离散条带。该方法可用于估计细胞或组织中聚（ADP-核糖）链的大小分布。