高分子量聚（二磷酸腺苷核糖）的证明

Tanaka M, Hayashi K, Sakura H, Miwa M, Matsushima T, Sugimura T

Nucleic Acids Res. 1978 Sep;5(9):3183-94. doi: 10.1093/nar/5.9.3183.

An electrophoretic system was established that resolves poly(adenosine diphosphate ribose), enzymatically synthesized polymer from NAD+, by size difference of one residue on polyacrylamide gel. The existence of a polymer of at least 65 residues was demonstrated by band counting in this system. The polymer showed a heterogeneous size distribution on the electrophoregram, and the molecular weight of the largest polymer was deduced to be more than 4.5 X 10(5) daltons. The discrepancy between the size, estimated by electrophoresis, and the chain length, determined by the ratio of total radioactivity to that derived from the terminus, suggests that the polymer has a branched structure.

建立了一种电泳系统，该系统通过聚丙烯酰胺凝胶上一个残基的大小差异来分离由烟酰胺腺嘌呤二核苷酸（NAD +）酶促合成的聚（腺苷二磷酸核糖）。通过该系统中的条带计数证明了至少65个残基的聚合物的存在。该聚合物在电泳图上显示出异质的大小分布，并且推断最大聚合物的分子量超过4.5×10（5）道尔顿。通过电泳估计的大小与由总放射性与末端衍生的放射性之比确定的链长度之间的差异表明该聚合物具有分支结构。