Tsukamoto K, Obata H, Hihara H
Poultry Disease Laboratory, National Institute of Animal Health, Gifu-ken, Japan.
Avian Dis. 1990 Jan-Mar;34(1):209-13.
Serotype 1 of infectious bursal disease virus (IBDV) adapted to chicken embryo fibroblasts (CEF) was used for the preparation of enzyme-linked immunosorbent assay (ELISA) antigen. After several passages of diluted viruses in CEF cultures, the titer of seed virus increased to 1.2 x 10(8) plaque-forming units/ml. Purified virus prepared from this seed virus had high titers of antigen and was less nonspecific than that from low titer of seed virus in an ELISA. The nonspecific reaction of purified virus decreased further after treatment with Triton X-100. When the specificity of this treated antigen was examined with specific-pathogen-free chicken sera before and during lay and with 14 antisera to some major avian viruses, this ELISA antigen had no nonspecific reaction and was specific to antibodies to serotypes 1 and 2 of IBDV.
将适应鸡胚成纤维细胞（CEF）的传染性法氏囊病病毒（IBDV）血清1型用于制备酶联免疫吸附测定（ELISA）抗原。在CEF培养物中对稀释病毒进行多次传代后，种毒滴度提高到1.2×10⁸蚀斑形成单位/毫升。用该种毒制备的纯化病毒具有高抗原滴度，并且在ELISA中比低滴度种毒制备的纯化病毒非特异性更低。用Triton X-100处理后，纯化病毒的非特异性反应进一步降低。当用产蛋前和产蛋期间的无特定病原体鸡血清以及14种针对一些主要禽病毒的抗血清检测这种处理后抗原的特异性时，这种ELISA抗原无非特异性反应，并且对IBDV血清1型和2型抗体具有特异性。
