Wu Kwou-Yeung, Wang Hwei-Zu, Hong Show-Jen
Department of Ophthalmology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
Kaohsiung J Med Sci. 2006 Aug;22(8):385-9. doi: 10.1016/S1607-551X(09)70327-4.
The purpose of this study is to investigate the effects of antibiotics and glucocorticoid eyedrops, including gentamicin, sulfisomezole, fluorometholone, dexamethasone, and betamethasone, on cellular proliferation in cultured human corneal keratocytes. Human corneal keratocytes were cultured in RPMI-1640 containing 10% fetal bovine serum. Drugs were prepared from original concentrations to 1/10, 1/100, and 1/1,000 dilutions. After exposure to drugs for 100 minutes, cellular proliferation was estimated by [3H]-thymidine uptake. It was found that cellular proliferation in corneal keratocytes was not affected by any of the three dilutions of gentamicin but was inhibited by 1/10 and 1/100 dilutions of sulfisomezole to 82% and 90% of control. [3H]-thymidine uptake values were inhibited to 75% by 1/10 dilution of fluorometholone and by 1/10and 1/100 dilutions of betamethasone to 84% and 86% of control. Meanwhile, cellular proliferation was significantly inhibited by 1/10, 1/100, and 1/1,000 dilutions of dexamethasone to 82%,86%, and 90%, respectively, in comparison with control values. It was demonstrated that commercial eyedrops of glucocorticoids inhibit cellular proliferation in corneal keratocytes, which may modulate the wound healing of corneal stroma.
本研究的目的是调查抗生素和糖皮质激素眼药水（包括庆大霉素、磺胺异噁唑、氟米龙、地塞米松和倍他米松）对培养的人角膜基质细胞增殖的影响。人角膜基质细胞在含有10%胎牛血清的RPMI-1640培养基中培养。将药物从原液稀释至1/10、1/100和1/1000倍。在药物作用100分钟后，通过[³H] - 胸腺嘧啶核苷摄取评估细胞增殖。结果发现，庆大霉素的三种稀释液均未影响角膜基质细胞的增殖，但磺胺异噁唑的1/10和1/100稀释液可将细胞增殖抑制至对照组的82%和90%。氟米龙的1/10稀释液可将[³H] - 胸腺嘧啶核苷摄取值抑制至对照组的75%，倍他米松的1/10和1/100稀释液可将其抑制至对照组的84%和86%。同时，与对照组相比，地塞米松的1/10、1/100和1/1000稀释液分别将细胞增殖显著抑制至82%、86%和90%。结果表明，市售糖皮质激素眼药水可抑制角膜基质细胞的增殖，这可能会调节角膜基质的伤口愈合。
