Tani Emiko, Katakami Chikako, Negi Akira
Division of Organ Therapeutics, Department of Ophthalmology, Kobe University Graduate School of Medicine, Japan.
Jpn J Ophthalmol. 2002 Sep-Oct;46(5):488-95. doi: 10.1016/s0021-5155(02)00541-5.
The effects of various eye drops on corneal wound healing, particularly in the subepithelial haze area, were investigated histologically following superficial keratectomy in rabbits.
Mechanical superficial keratectomy was performed in rabbit eyes. Tranilast, betamethasone, hyaluronic acid, and diclofenac eye drops were administered after the procedure. Physiological saline was used as a control. Corneas were excised 1, 2, 3, and 4 weeks after keratectomy, labeled with 3H-thymidine or 3H-proline, and subjected to autoradiography.
In the control and diclofenac groups, corneal haze occurred 3 weeks after keratectomy. Histological examination revealed an accumulation of proliferating keratocytes and active synthesis of collagen in the subepithelial area. In the tranilast and betamethasone groups, formation of corneal haze was reduced compared to the controls. The proliferation of keratocytes and the production of collagen in the corneal stroma were inhibited by these drugs. In the hyaluronic acid group also, corneal haze was decreased. In this group, although the proliferation of keratocytes was activated compared to the controls, abnormal accumulation of keratocytes in the subepithelial area was not detected.
Tranilast and betamethasone decrease the formation of subepithelial haze by inhibiting keratocyte proliferation and synthesis of extracellular matrix in the corneal stroma. Hyaluronic acid, on the other hand, inhibits subepithelial haze by promoting physiologic wound healing.
通过对兔浅表角膜切除术后的组织学研究，探讨各种滴眼液对角膜伤口愈合的影响，特别是对上皮下混浊区域的影响。
对兔眼进行机械性浅表角膜切除术。术后分别给予曲尼司特、倍他米松、透明质酸和双氯芬酸滴眼液。以生理盐水作为对照。在角膜切除术后1、2、3和4周切除角膜，用³H-胸腺嘧啶核苷或³H-脯氨酸标记，然后进行放射自显影。
在对照组和双氯芬酸组中，角膜混浊在角膜切除术后3周出现。组织学检查显示上皮下区域增殖性角膜细胞积聚和胶原的活跃合成。在曲尼司特组和倍他米松组中，与对照组相比角膜混浊的形成减少。这些药物抑制了角膜基质中角膜细胞的增殖和胶原的产生。在透明质酸组中，角膜混浊也有所减轻。在该组中，虽然与对照组相比角膜细胞的增殖被激活，但未检测到上皮下区域角膜细胞的异常积聚。
曲尼司特和倍他米松通过抑制角膜基质中角膜细胞的增殖和细胞外基质的合成来减少上皮下混浊的形成。另一方面，透明质酸通过促进生理性伤口愈合来抑制上皮下混浊。
