Hiraoka Koji, Grogan Shawn, Olee Tsaiwei, Lotz Martin
Division of Arthritis Research, The Scripps Research Institute, La Jolla, CA 92037, USA.
Biorheology. 2006;43(3,4):447-54.
The transmembrane receptor Notch-1 regulates cell fate and differentiation and was suggested to identify a cell type with progenitor characteristics in newborn bovine articular cartilage. We show that Notch-1 is expressed on > 70% of BM-MSC in early passage monolayer culture. We also demonstrate that normal articular cartilage contains Notch-1+ cells and that the frequency is increased in OA. Most Notch-1+ cells in OA cartilage are located in the clusters of proliferating cells. These findings indicate that multipotential mesenchymal progenitor cells are present in articular cartilage from adult humans and that their frequency is increased in OA. This observation has implications for understanding the intrinsic repair capacity of articular cartilage and raises the possibility that these progenitor cells might be involved in the pathogenesis of arthritis.
跨膜受体Notch-1调节细胞命运和分化，有人提出它可识别新生牛关节软骨中具有祖细胞特征的细胞类型。我们发现，在早期传代的单层培养中，超过70%的骨髓间充质干细胞（BM-MSC）表达Notch-1。我们还证明，正常关节软骨含有Notch-1阳性细胞，且在骨关节炎（OA）中该频率增加。OA软骨中的大多数Notch-1阳性细胞位于增殖细胞簇中。这些发现表明，多能间充质祖细胞存在于成人关节软骨中，且在OA中其频率增加。这一观察结果对于理解关节软骨的内在修复能力具有重要意义，并增加了这些祖细胞可能参与关节炎发病机制的可能性。
