Université de Paris, Institut de Recherche Saint-Louis, EMiLy, INSERM U1160, Paris, France.
Université de Paris, Institut de Recherche Saint-Louis, OPALE Carnot Institute, EMiLy, INSERM U1160, Paris, France.
Methods Mol Biol. 2021;2308:35-46. doi: 10.1007/978-1-0716-1425-9_3.
Mesenchymal stem/stromal cells (MSCs) are multipotent adult cells that are present in several tissues including the bone marrow (BM), in which they can differentiate in a variety of cell types such as osteoblasts, chondrocytes and adipocytes. The isolation of MSCs has been carried out by many studies that aim to control their differentiation into cartilaginous and bone cells in vitro in order to use this technology in the repair of damaged tissues. Here we describe the minimum requirements and an efficient method for isolation, expansion of mouse bone-derived multipotent mesenchymal stromal cells and their differentiation into osteoblasts, responsible for the bone matrix synthesis and mineralization.
间充质干细胞（MSCs）是多能成体细胞，存在于多种组织中，包括骨髓（BM），在其中它们可以分化为多种细胞类型，如成骨细胞、软骨细胞和脂肪细胞。许多研究已经进行了 MSCs 的分离，旨在控制它们在体外向软骨和骨细胞分化，以便将这项技术用于修复受损组织。在这里，我们描述了分离、扩增小鼠骨源多能间充质基质细胞及其分化为成骨细胞的最低要求和有效方法，成骨细胞负责骨基质的合成和矿化。
