Bindayna K M, Al Baker W A, Botta G A
Department of Microbiology, Immunology and Infectious Diseases, College of Medicine and Medical Sciences, Arabian Gulf University, Kingdom of Bahrain.
Indian J Med Microbiol. 2006 Jul;24(3):195-200.
Helicobacter pylori infection is common in the developing countries. The cagA gene is a marker of pathogenicity island (PAI) in H. pylori . The aim of this study was to determine the prevalence of cagA among dyspeptic patients in Bahrain directly from gastric biopsy and stool specimen.
A total of 100 gastric biopsy samples, 16 clinical isolates and 44 faecal specimens were collected from Bahraini adult dyspeptic patients. cagA gene of H. pylori was assessed using polymerase chain reaction (PCR).
The cagA gene was detected in 59 (59%) from biopsy specimens, 10 (62%) clinical isolates and in 10 (22.7%) faecal specimens. The detection of cagA positive H. pylori was significantly higher in patients with duodenal ulcer (80%) compared to those with other endoscopic finding (42%) (P < 0.05).
Using PCR to detect cagA gene directly from biopsy is a rapid and reliable technique. However, using stool specimen for genotyping in our patients showed reduced sensitivity.
幽门螺杆菌感染在发展中国家很常见。cagA基因是幽门螺杆菌致病岛（PAI）的一个标志物。本研究的目的是直接从胃活检和粪便标本中确定巴林消化不良患者中cagA的流行情况。
从巴林成年消化不良患者中总共收集了100份胃活检样本、16份临床分离株和44份粪便标本。使用聚合酶链反应（PCR）评估幽门螺杆菌的cagA基因。
在59份（59%）活检标本、10份（62%）临床分离株和10份（22.7%）粪便标本中检测到了cagA基因。十二指肠溃疡患者中cagA阳性幽门螺杆菌的检出率（80%）显著高于其他内镜检查结果患者（42%）（P<0.05）。
使用PCR直接从活检中检测cagA基因是一种快速可靠的技术。然而，在我们的患者中使用粪便标本进行基因分型显示敏感性降低。
