A Cross-Sectional Study on Molecular Detection of cytotoxin-associated gene A and 16SrRNA Gene from Gastric Biopsy Specimens.

INTRODUCTION

The aim of the study is relative proportion of cytotoxin-associated gene A () virulence marker in isolates and gastric biopsy samples by polymerase chain reaction (PCR).

METHODS

This cross-sectional study was conducted at a tertiary care hospital setting. Gastric biopsy tissues from 200 patients, suffering from upper gastrointestinal tract disorders, were examined for infection using methods, such as hematoxylin and eosin (H and E) staining, (), and gene PCR. Chi-square and kappa statistics were used to find the association and agreement between the tests, respectively; ≤ 0.05 was considered statistically significant. Screening tests' accuracy was calculated in terms of sensitivity and specificity along with positive and negative predictive values.

RESULTS

Out of 200 patients, was detected in 14.5%, 48.5%, and 31% patients by H and E staining, 16S rRNA, and PCR, respectively. Sensitivity and specificity of PCR as compared to H and E staining were 89.6% and 78.9%, respectively.

CONCLUSIONS

detection directly from biopsy specimen by PCR can potentially and rapidly determine the patient's status, especially when at a higher risk of peptic ulcer.