Yim Jae-Joon, Adams Amelia A, Kim Ju Han, Holland Steven M
Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine and Lung Institute of Medical Research Center, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, Seoul, Republic of Korea.
Immunogenetics. 2006 Sep;58(9):740-5. doi: 10.1007/s00251-006-0141-2. Epub 2006 Aug 16.
Nonhuman primates express varying responses to Mycobacterium tuberculosis: New World monkeys appear to be resistant to tuberculosis (TB) while Old World monkeys seem to be particularly susceptible. The aim of this study was to elucidate the presence of the regulatory guanine-thymine (GT) repeat polymorphisms in intron 2 of Toll-like receptor 2 (TLR2) associated with the development of TB in humans and to determine any variations in these microsatellite polymorphisms in primates. We sequenced the region encompassing the regulatory GT repeat microsatellites in intron 2 of TLR2 in 12 different nonhuman primates using polymerase chain reaction amplification, TA cloning, and automatic sequencing. The nonhuman primates included for this study were as follows: chimpanzee (Pan troglodytes), bonobo (Pan paniscus), gorilla (Gorilla gorilla), orangutan (Pongo pygmaeus), Celebes ape (Macaca nigra), rhesus monkey (Macaca mulatta), pigtail macaque (Macaca nemestrina), patas monkey (Erythrocebus patas), spider monkey (Ateles geoffroyi), Woolly monkey (Lagothrix lagotricha), tamarin (Saguinus labiatus), and ring-tailed lemur (Lemur catta). Nucleotide sequences encompassing the regulatory GT repeat region are similar across species and are completely conserved in great apes. However, Old World monkeys lack GT repeats altogether, while New World monkeys and ring-tailed lemurs have much more complex structures around the position of the repeats. In conclusion, the genetic structures encompassing the regulatory GT repeats in intron 2 of human TLR2 are similar among nonhuman primates. The sequence is most conserved in New World monkeys and less in Old World monkeys.
新大陆猴似乎对结核病（TB）具有抗性，而旧大陆猴似乎特别易感。本研究的目的是阐明人类中与结核病发生相关的Toll样受体2（TLR2）内含子2中调控性鸟嘌呤-胸腺嘧啶（GT）重复多态性的存在，并确定这些微卫星多态性在灵长类动物中的任何变异。我们使用聚合酶链反应扩增、TA克隆和自动测序技术，对12种不同的非人灵长类动物TLR2内含子2中包含调控GT重复微卫星的区域进行了测序。本研究纳入的非人灵长类动物如下：黑猩猩（Pan troglodytes）、倭黑猩猩（Pan paniscus）、大猩猩（Gorilla gorilla）、猩猩（Pongo pygmaeus）、西里伯斯猿（Macaca nigra）、恒河猴（Macaca mulatta）、猪尾猕猴（Macaca nemestrina）、赤猴（Erythrocebus patas）、蜘蛛猴（Ateles geoffroyi）、绒毛猴（Lagothrix lagotricha）、狨猴（Saguinus labiatus）和环尾狐猴（Lemur catta）。跨物种的包含调控GT重复区域的核苷酸序列相似，并且在大猩猩中完全保守。然而，旧大陆猴完全缺乏GT重复，而新大陆猴和环尾狐猴在重复位置周围具有更为复杂的结构。总之，人类TLR2内含子2中包含调控GT重复的遗传结构在非人灵长类动物中相似。该序列在新大陆猴中最保守，在旧大陆猴中较不保守。
