Jin Shumei, Cheng Yuxiang, Guan Qingjie, Liu Dali, Takano Tetsuo, Liu Shenkui
Alkali Soil Natural Environmental Science Center (ASNESC), Stress Molecular Biology Laboratory, Northeast Forestry University, Harbin 150040, P. R. China.
Biotechnol Lett. 2006 Nov;28(21):1749-53. doi: 10.1007/s10529-006-9152-1. Epub 2006 Aug 16.
A metallothionein-like (rgMT) gene was isolated from a rice (Oryza sativa L.) root cDNA library that was prepared from plants grown under NaHCO3 stress. The rgMT gene expression was induced in rice leaves and roots under several abiotic stresses from salts (NaCl and NaHCO3), drought (PEG) and metals (CuCl2, ZnCl2, CdCl2). The results suggested that the rgMT gene was expressed in response to environmental stresses. The rgMT gene was expressed in Escherichia coli, and the final yield of the purified rgMT protein was 4.8 mg g(-1) dry cells. Tolerance of E. coli expressing GST-rgMT fusion protein to Cu2+, Zn2+ and Cd2+ was enhanced, and cells dry weight increased 0.04 mg, 0.17 mg and 0.07 mg in 1 ml culture treated with either CuCl2, ZnCl2 or CdCl2, respectively, compared with control after 6 h culture.
从在NaHCO₃胁迫下生长的水稻（Oryza sativa L.）根cDNA文库中分离出一个类金属硫蛋白（rgMT）基因。在盐（NaCl和NaHCO₃）、干旱（PEG）和金属（CuCl₂、ZnCl₂、CdCl₂）等几种非生物胁迫下，rgMT基因在水稻叶片和根中被诱导表达。结果表明，rgMT基因是响应环境胁迫而表达的。rgMT基因在大肠杆菌中表达，纯化后的rgMT蛋白最终产量为4.8 mg g⁻¹干细胞。表达GST-rgMT融合蛋白的大肠杆菌对Cu²⁺、Zn²⁺和Cd²⁺的耐受性增强，与对照相比，在1 ml培养物中分别用CuCl₂、ZnCl₂或CdCl₂处理6小时后，细胞干重分别增加了0.04 mg、0.17 mg和0.07 mg。
