Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin, 150040, China.
Institute of Maize, Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, China.
Sci Rep. 2017 Dec 20;7(1):17914. doi: 10.1038/s41598-017-18263-4.
A type 2 metallothionein gene, SsMT2, was cloned from Suaeda salsa, a salt- and alkali-tolerant plant, which is dominant species on the saline/alkali soil of northeast China. The SsMT2 gene was expressed in all organs except the flower and its expression was induced by various stresses such as CdCl, NaCl, NaHCO, and HO treatments. SsMT2-transgenic yeast (Saccharomyces cerevisiae) and plants (Arabidopsis thaliana) showed significantly increased resistance to metal, salt and oxidant stresses. These transgenics accumulated more Cd, but less Na than their wild type counterparts. SsMT2 transgenic Arabidopsis maintained lower level of HO than wild type plants did in response to the stress treatments. These results demonstrated that the SsMT2 gene plays an important role in reactive oxygen species scavenging and confers enhanced metal and oxidant tolerance to plants.
从耐盐碱性植物盐地碱蓬中克隆到一个 2 型金属硫蛋白基因 SsMT2，该基因在除花以外的所有器官中均有表达，并且其表达受到 CdCl2、NaCl、NaHCO3 和 H2O2 等各种胁迫的诱导。SsMT2 转基因酵母（酿酒酵母）和植物（拟南芥）对金属、盐和氧化剂胁迫表现出显著增强的抗性。这些转基因植物积累的 Cd 多于 Na，而野生型植物则相反。在应对胁迫处理时，SsMT2 转基因拟南芥的 H2O2 水平低于野生型植物。这些结果表明，SsMT2 基因在清除活性氧方面发挥着重要作用，并赋予植物对金属和氧化剂更强的耐受性。
