Iwamoto T, Takahashi M, Ito M, Hamaguchi M, Isobe K, Misawa N, Asai J, Yoshida T, Nakashima I
Department of Immunology, Nagoya University School of Medicine, Japan.
Oncogene. 1990 Apr;5(4):535-42.
We have successfully produced transgenic mice that carry the ret oncogene driven by a mouse mammary tumor virus promoter/enhancer. Mammary and salivary gland adenocarcinomas were developed in a stochastic fashion in these mice. Moreover, premalignant tumors with hyperplastic and dysplastic lesions of Harderian glands and male reproductive tracts frequently occurred at young ages. High expression of the transgene was closely associated with the development of these tumors although the levels of the transgene expression were variable among individuals. In addition, large amounts of phosphotyrosine-containing proteins were detected in cell lysates from mammary and salivary adenocarcinomas by immunoblotting with the anti-phosphotyrosine antibody.
我们成功培育出了携带由小鼠乳腺肿瘤病毒启动子/增强子驱动的ret癌基因的转基因小鼠。在这些小鼠中，乳腺和唾液腺腺癌以随机方式发生。此外，哈氏腺和雄性生殖道具有增生性和发育异常性病变的癌前肿瘤在年轻时经常出现。尽管转基因表达水平在个体间存在差异，但转基因的高表达与这些肿瘤的发生密切相关。此外，通过用抗磷酸酪氨酸抗体进行免疫印迹，在乳腺和唾液腺癌的细胞裂解物中检测到大量含磷酸酪氨酸的蛋白质。
