Iwamoto T, Taniguchi M, Wajjwalku W, Nakashima I, Takahashi M
Department of Immunology, Nagoya University School of Medicine, Japan.
Br J Cancer. 1993 Mar;67(3):504-7. doi: 10.1038/bjc.1993.94.
We have recently succeeded in producing transgenic mice carrying a hybrid gene consisting of mouse metallothionein promoter-enhancer and the ret oncogene (MT/ret). (Iwamoto et al., 1991b). A retroperitoneal tumour developed in one of 17 MT/ret transgenic founder mice. Histological analysis revealed that the tumour consisted of undifferentiated neuroblasts and differentiated ganglion cells, the latter of which were strongly positive for neuron specific enolase. Expression of the ret transgene was observed at high levels in RNA from the tumour, but not in those of other normal tissues. In addition, a 100kDa ret protein was detected in the cell lysate of the tumour. Taken together with our previous data, these results suggest a possible role for the ret oncogene in the proliferation of neural crest cells.
我们最近成功培育出了携带由小鼠金属硫蛋白启动子-增强子和ret癌基因组成的杂交基因（MT/ret）的转基因小鼠。（岩本等人，1991b）。在17只MT/ret转基因奠基小鼠中的一只身上出现了腹膜后肿瘤。组织学分析显示，该肿瘤由未分化的神经母细胞和分化的神经节细胞组成，后者对神经元特异性烯醇化酶呈强阳性。在肿瘤的RNA中观察到ret转基因的高水平表达，但在其他正常组织的RNA中未观察到。此外，在肿瘤的细胞裂解物中检测到一种100kDa的ret蛋白。结合我们之前的数据，这些结果表明ret癌基因在神经嵴细胞增殖中可能发挥作用。
