Ciapina Elisa M P, Melo Walber C, Santa Anna Lidia M M, Santos Alexandre S, Freire Denise M G, Pereira Júnior Nei
Departamento de Bioquímica, Universidade Federal do Rio de Janeiro, Centro de Tecnologia, Bloco E, Rio de Janeiro, RJ, CEP 21949-900, Brasil.
Appl Biochem Biotechnol. 2006 Spring;129-132:880-6.
The production of biosurfactant by Rhodococcus erythropolis during the growth on glycerol was investigated. The process was carried out at 28 degrees C in a 1.5-L bioreactor using glycerol as carbon source. The bioprocess was monitored through measurements of biosurfactant concentration and glycerol consumption. After 51 h of cultivation, 1.7 g/L of biosurfactant, surface, and interfacial tensions values (with n-hexadecane) of 43 and 15 mN/m, respectively, 67% of Emulsifying Index (E24), and 94% of oil removal were obtained. The use of glycerol rather than what happens with hydrophobic carbon source allowed the release of the biosurfactant, originally associated to the cell wall.
研究了红平红球菌在以甘油为碳源生长过程中生物表面活性剂的产生情况。该过程在28℃下于1.5升生物反应器中进行，使用甘油作为碳源。通过测量生物表面活性剂浓度和甘油消耗来监测生物过程。培养51小时后，获得了1.7克/升的生物表面活性剂，其表面张力和界面张力值（与正十六烷）分别为43和15毫牛/米，乳化指数（E24）为67%，除油率为94%。使用甘油而非疏水性碳源的情况使得原本与细胞壁相关的生物表面活性剂得以释放。
