Gogotov I N, Khodakov R S
Prikl Biokhim Mikrobiol. 2008 Mar-Apr;44(2):207-12.
It has been shown that the Rhodococcus erythropolis sH-5 strain can produce surfactants associated and not associated with the cell wall. Their content depends on medium composition, the nature of the carbon source, and oxygen supply. The highest biosurfactant (bioSF) yield is achieved by growing R. erythropolis sH-5 in medium with 2% kerosene at neutral pH. It has been found that the bioSF yield and emulsification index for various hydrocarbons depend on the kind of the nitrogen source used by the bacterium, increasing with replacement of KNO3 by NaNO3. The yields of biomass and bioSF in R. erythropolis depend on growth temperatures (max at 30 degrees C) but not on water quality (bidistillate, catholyte, or anolyte). It has been found that sH-5 produces more cell-associated bioSF than extracellular species.
已表明红平红球菌sH-5菌株可产生与细胞壁相关和不相关的表面活性剂。它们的含量取决于培养基组成、碳源性质和氧气供应。通过在中性pH值下含2%煤油的培养基中培养红平红球菌sH-5，可实现最高的生物表面活性剂（bioSF）产量。已发现，各种烃类的bioSF产量和乳化指数取决于细菌所用氮源的种类，用NaNO3替代KNO3时会增加。红平红球菌中生物量和bioSF的产量取决于生长温度（30℃时最高），但不取决于水质（双蒸馏水、阴极电解液或阳极电解液）。已发现sH-5产生的与细胞相关的bioSF比细胞外种类更多。
