Srigley J R, Dardick I, Hartwick R W, Klotz L
Department of Pathology, Sunnybrook Medical Centre, University of Toronto, Canada.
Am J Pathol. 1990 Apr;136(4):957-66.
The hypothesis that basal epithelial cells of the human prostate are of myoepithelial origin was investigated using immunohistochemical and ultrastructural methodologies. The immunohistologic analyses show significant phenotypic differences between prostatic basal cells and myoepithelial cells of the salivary gland. Although both cell types stain intensely with the 312C8-1 monoclonal antibody, only true myoepithelial cells demonstrated significant amounts of muscle-specific actin as decorated by the HHF35 monoclonal antibody. Furthermore, using double-labeling experiments, the prostatic basal cells were strongly decorated with a fluorescein-tagged basal cell-specific keratin but were negative with the rhodamine-tagged phalloidin, a chemical that binds specifically to actin microfilaments. Ultrastructural studies also showed an absence of thin microfilament bundles, dense bodies, and micropinocytotic vesicles in the prostatic basal cells. The current investigations show that the prostatic acini do not have a basal myoepithelium. Although some authors have suggested a stem cell role for prostatic basal cells, the weight of experimental work argues against this hypothesis. The exact role of the basal epithelial cells of the prostate is not known, although they may serve endocrine, paracrine, or other regulatory functions and may be involved in modulating signals between prostatic stroma and epithelium.
采用免疫组织化学和超微结构方法，对人前列腺基底上皮细胞起源于肌上皮细胞的假说进行了研究。免疫组织学分析显示，前列腺基底细胞与唾液腺肌上皮细胞之间存在显著的表型差异。尽管两种细胞类型均被312C8-1单克隆抗体强烈染色，但只有真正的肌上皮细胞显示出大量由HHF35单克隆抗体标记的肌肉特异性肌动蛋白。此外，通过双标记实验，前列腺基底细胞被荧光素标记的基底细胞特异性角蛋白强烈标记，但被罗丹明标记的鬼笔环肽（一种特异性结合肌动蛋白微丝的化学物质）标记为阴性。超微结构研究还显示，前列腺基底细胞中不存在细肌丝束、致密体和微吞饮小泡。目前的研究表明，前列腺腺泡没有基底肌上皮。尽管一些作者认为前列腺基底细胞具有干细胞作用，但大量实验工作反对这一假说。前列腺基底上皮细胞的确切作用尚不清楚，尽管它们可能发挥内分泌、旁分泌或其他调节功能，并可能参与调节前列腺基质与上皮之间的信号。
