Suzuki Y, Aoyagi Y, Muramatsu M, Igarashi K, Saito A, Oguro M, Isemura M, Asakura H
Department of Internal Medicine, Niigata University School of Medicine, Japan.
Ann Clin Biochem. 1990 Mar;27 ( Pt 2):121-8. doi: 10.1177/000456329002700206.
Purified alpha-fetoprotein (AFP), fucosylated AFP mixtures, and 40 sera from patients with AFP-producing hepatocellular carcinoma were analysed by monoclonal enzyme immunoassay (EIA) to distinguish fucosylated and nonfucosylated AFP molecular variants. FUC-AFP-25 was discriminated from FUC-AFP-75 by the EIA using monoclonal antibody 18H4 in the range of total AFP concentrations from 100 to 800 ng/mL. In addition, sera from 40 patients with hepatocellular carcinoma, with AFP concentrations from 100 to 1270 ng/mL and with fucosylated AFP from 0 to 100% by conventional cross immuno-affinoelectrophoresis, were also analysed by the present EIA. A statistically significant correlation was obtained between the data from the present EIA and from the conventional crossed immuno-affinoelectrophoresis in the range of fucosylated AFP more than 20% and the serum concentration of AFP more than 100 ng/mL. These results indicate that the present EIA is useful for clinical detection of hepatocellular carcinoma during the follow-up of patients with chronic liver diseases.
采用单克隆酶免疫测定法（EIA）对纯化的甲胎蛋白（AFP）、岩藻糖基化AFP混合物以及40例产生AFP的肝细胞癌患者的血清进行分析，以区分岩藻糖基化和非岩藻糖基化的AFP分子变体。在总AFP浓度为100至800 ng/mL的范围内，使用单克隆抗体18H4的EIA可区分FUC-AFP-25和FUC-AFP-75。此外，还采用本EIA对40例肝细胞癌患者的血清进行了分析，这些患者的AFP浓度为100至1270 ng/mL，通过传统交叉免疫亲和电泳法测得的岩藻糖基化AFP含量为0至100%。在岩藻糖基化AFP含量超过20%且血清AFP浓度超过100 ng/mL的范围内，本EIA数据与传统交叉免疫亲和电泳数据之间存在统计学显著相关性。这些结果表明，本EIA对于慢性肝病患者随访期间肝细胞癌的临床检测具有重要意义。
