Zaninotto M, Ujka F, Lachin M, Bernardi D, Marafi C, Farinati F, Plebani M
Laboratorio Centrale, Azienda Ospedaliera di Padova, Italy.
Anticancer Res. 1996 Jan-Feb;16(1):305-9.
Our aims were to ascertain the analytical performance and diagnostic efficiency of a new lectin-affinity electrophoresis and antibody-affinity blotting method for separating and identifying fucosylated AFP. We also compared the diagnostic efficiency of fucosylated AFP with that of total AFP in the early diagnosis of neoplastic liver diseases. In 32 patients with hepatocellular carcinoma (HCC) and in 19 subjects with benign liver diseases, total AFP was assayed using an enzyme immunoassay, while AFP fractions (L1, L2 and to cathode L3 fucosylated) were separated using lectin-affinity electrophoresis followed by antibody-affinity blotting. No significant difference was found between the total AFP values of patients with HCC and those of patients with benign liver disease (p > 0.05). In patients with HCC the fucosylation index of AFP (L3%) was significantly higher (p < 0.0001) than that in patients with benign liver disease. Our findings suggest that fucosylated AFP is of diagnostic value in differentiating between hepatocellular carcinoma and benign hepatic diseases, and that its measurement may be useful in the early detection of hepatocarcinoma in patients with chronic liver disease.
我们的目的是确定一种用于分离和鉴定岩藻糖基化甲胎蛋白的新型凝集素亲和电泳和抗体亲和印迹法的分析性能和诊断效率。我们还比较了岩藻糖基化甲胎蛋白与总甲胎蛋白在肿瘤性肝病早期诊断中的诊断效率。对32例肝细胞癌（HCC）患者和19例良性肝病患者，采用酶免疫测定法检测总甲胎蛋白，同时用凝集素亲和电泳和抗体亲和印迹法分离甲胎蛋白组分（L1、L2和向阴极的L3岩藻糖基化组分）。HCC患者的总甲胎蛋白值与良性肝病患者的总甲胎蛋白值之间未发现显著差异（p>0.05）。HCC患者甲胎蛋白的岩藻糖基化指数（L3%）显著高于良性肝病患者（p<0.0001）。我们的研究结果表明，岩藻糖基化甲胎蛋白在区分肝细胞癌和良性肝病方面具有诊断价值，其检测可能有助于慢性肝病患者肝癌的早期发现。
