Aoyagi Y, Isemura M, Yosizawa Z, Suzuki Y, Sekine C, Ono T, Ichida F
Biochim Biophys Acta. 1985 Aug 23;830(3):217-23. doi: 10.1016/0167-4838(85)90277-8.
alpha-Fetoprotein specimens were prepared from the sera of four patients with hepatocellular carcinoma. The lentil lectin-reactive and lectin-nonreactive variants of this glycoprotein were also prepared from the serum of one of the four patients by affinity chromatography with immobilized lectin. The correlation between the carbohydrate structure of these compounds and their reactivity in crossed immuno-affinoelectrophoresis with lentil lectin was studied by chemical analysis and affinity chromatography of the glycopeptides with lectin columns. It was found that the lentil lectin-reactive variant contained a carbohydrate chain of the fucosylated biantennary complex type. These data together with previous findings indicate that most of the patients with hepatocellular carcinoma have an elevated serum concentration of fucosylated alpha-fetoprotein.
从四名肝细胞癌患者的血清中制备了甲胎蛋白标本。还通过使用固定化凝集素的亲和色谱法从这四名患者之一的血清中制备了这种糖蛋白的扁豆凝集素反应性和非凝集素反应性变体。通过糖肽与凝集素柱的化学分析和亲和色谱法，研究了这些化合物的碳水化合物结构与其在与扁豆凝集素的交叉免疫亲和电泳中的反应性之间的相关性。发现扁豆凝集素反应性变体含有岩藻糖基化双天线复合型的碳水化合物链。这些数据与先前的研究结果表明，大多数肝细胞癌患者的血清中岩藻糖基化甲胎蛋白浓度升高。
