Goncharov Nikolai, Katsya Gulinara, Dobracheva Anna, Nizhnik Alexander, Kolesnikova Galina, Herbst Victor, Westermann Jürgen
National Center of Endocrinology, Dm. Ulyanova Street, 11, 117036, Moscow, Russia.
Aging Male. 2006 Jun;9(2):111-22. doi: 10.1080/13685530600713060.
The accurate measurement of testosterone remains a challenge. The determination of the blood testosterone concentrations in serum by conventional immunoassays is inaccurate in men and even more so in females and children. A new luminescence enzyme immunoassay (LIA) has been developed and validated. The high analytical (8.7 pmol/L) and functional (17.3 pmol/L) sensitivity allows the quantification of the very low concentration in saliva, as well as in serum, after 1/40 dilution. This study measured salivary testosterone levels and compared the results with the free levels calculated from total testosterone and sex hormone-binding globulin in eugonadal and hypogonadal men. Salivary testosterone concentrations in healthy men in morning hours were 369 pmol/L (mean), range 263-544 pmol/L, which was statistically significantly higher than that in men with androgen deficiency, 215 pmol/L (mean), range 51-249 pmol/L. Repetitive determination of free testosterone concentrations in saliva (once a week for 5 weeks) showed high stability of results over time, with coefficient of variation 9% (range 5-23%). In this study we showed that free salivary testosterone levels in morning samples correlated well with calculated free testosterone in blood, both in healthy men (R = 0.754, P = 0.001), and in patients with androgen deficiency (R = 0.889, P = 0.0001), though in cases with very low testosterone, salivary concentrations were systematically higher than calculated free testosterone levels in blood.
睾酮的准确测量仍是一项挑战。通过传统免疫测定法测定血清中的血睾酮浓度，对于男性而言并不准确，对于女性和儿童更是如此。一种新的发光酶免疫测定法（LIA）已被开发并验证。其高分析灵敏度（8.7 pmol/L）和功能灵敏度（17.3 pmol/L）使得在1/40稀释后，能够对唾液以及血清中的极低浓度进行定量。本研究测量了唾液睾酮水平，并将结果与性腺功能正常和性腺功能减退男性中根据总睾酮和性激素结合球蛋白计算出的游离水平进行比较。健康男性早晨唾液睾酮浓度为369 pmol/L（均值），范围为263 - 544 pmol/L，这在统计学上显著高于雄激素缺乏男性的浓度，后者为215 pmol/L（均值），范围为51 - 249 pmol/L。对唾液中游离睾酮浓度进行重复测定（连续5周每周一次）显示，随着时间推移结果具有高稳定性，变异系数为9%（范围为5 - 23%）。在本研究中我们表明，早晨样本中唾液游离睾酮水平与血液中计算出的游离睾酮水平在健康男性（R = 0.754，P = 0.001）和雄激素缺乏患者（R = 0.889，P = 0.0001）中均具有良好的相关性，不过在睾酮水平极低的情况下，唾液浓度系统性地高于血液中计算出的游离睾酮水平。
