Morris Andrew P, Tawil Ahmad, Berkova Zuzana, Wible Linda, Smith C Wayne, Cunningham Sonia A
Department of Integrative Biology and Pharmacology, University of Texas at Houston Medical School, Houston, Texas 77030, USA.
Cell Commun Adhes. 2006 Jul-Aug;13(4):233-47. doi: 10.1080/15419060600877978.
Junctional Adhesion Molecules (JAMs) are components and regulators of the well-characterized epithelial and endothelial tight junction. Since the molecular components of native fibroblast adherens-like junctions remain poorly described we determined JAM expression profiles in fibroblasts. We found JAM-C on human dermal, lung, and corneal primary fibroblast cultures. Within murine lines, JAM-A was found in L-cells, JAM-C in 3T3 L1 cells, and both JAM-A and JAM-C were co-expressed in NIH 3T3 fibroblasts. In primary dermal fibroblasts, JAM-C concentrated at zipper-like junctions that formed between apposing cells. Dual immunostaining showed JAM-C co-localization with the ZO-1 intracellular scaffolding molecule at cell contacts that ranged from 7 microm to over 25 microm in length. JAM-C also labeled similar zipper-like junctions detected with N-Cadherin and Cadherin-11 antibodies. We conclude that endogenous JAM-C is an integral component of the dermal fibroblast adherens-like junction, and our data extend the expression and potential function of JAMs into mesenchymal tissues.
连接黏附分子（JAMs）是特征明确的上皮和内皮紧密连接的组成成分及调节因子。由于天然成纤维细胞黏附样连接的分子成分仍描述甚少，我们测定了成纤维细胞中JAM的表达谱。我们在人真皮、肺和角膜原代成纤维细胞培养物中发现了JAM-C。在小鼠细胞系中，在L细胞中发现了JAM-A，在3T3 L1细胞中发现了JAM-C，并且在NIH 3T3成纤维细胞中JAM-A和JAM-C共表达。在原代真皮成纤维细胞中，JAM-C集中在相邻细胞之间形成的拉链样连接处。双重免疫染色显示，在长度从7微米到超过25微米的细胞接触处，JAM-C与ZO-1细胞内支架分子共定位。JAM-C还用N-钙黏蛋白和钙黏蛋白-11抗体检测到的类似拉链样连接进行了标记。我们得出结论，内源性JAM-C是真皮成纤维细胞黏附样连接的一个组成部分，并且我们的数据将JAMs的表达和潜在功能扩展到了间充质组织。
