Fidler R, Osipova T A, Grigor'ian A L, Papazov I P, Verbitskiĭ M Sh, Bulatov A A
Probl Endokrinol (Mosk). 1990 Jan-Feb;36(1):75-8.
Two stable hybridomas producing antibodies (Mab 1 and Mab 2) to bovine prolactin and belonging to the IgG1 subclass have been prepared. The cross-reactivity of Mab 1 and Mab 2 with some structurally similar pituitary protein (human, pig, whale, rat prolactins, bovine and human somatotropins) using indirect immunoenzymatic assay, was studied. It has been shown that Mab 2 reacts specifically only with bovine prolactin whereas Mab 1 interacts with human prolactin and prolactins of different animals. The specificity of Mab 1 to human prolactin was confirmed by immunoradiodetection assay on nitrocellulose filters. The data obtained give evidence of the existence of at least two different sterically nonoverlapping epitopes: one of them is specific exclusively for bovine prolactin and the other one is common, i.e. extraspecific.
已制备出两种稳定的杂交瘤，它们产生针对牛催乳素的抗体（单克隆抗体1和单克隆抗体2），且属于IgG1亚类。使用间接免疫酶测定法研究了单克隆抗体1和单克隆抗体2与一些结构相似的垂体蛋白（人、猪、鲸、大鼠催乳素，牛和人生长激素）的交叉反应性。结果表明，单克隆抗体2仅与牛催乳素特异性反应，而单克隆抗体1与人催乳素及不同动物的催乳素相互作用。通过在硝酸纤维素滤膜上进行免疫放射检测分析，证实了单克隆抗体1对人催乳素的特异性。所获得的数据证明至少存在两个不同的空间上不重叠的表位：其中一个仅对牛催乳素具有特异性，另一个是共同的，即非特异性的。
