Dupont D, Labiau S, Grosclaude J, Collin J C
I.N.R.A., Station de Recherches en Technologie et Analyses Laitières, Poligny, France.
Hybridoma. 1994 Dec;13(6):543-7. doi: 10.1089/hyb.1994.13.543.
A collection of monoclonal antibodies was raised against bovine plasminogen by immunizing mice with purified plasminogen. More than 300 positive clones were obtained in 1 fusion experiment (1300 hybrids tested). Four types of antibodies were characterized through their relative binding to plasmin and plasminogen in ELISA. About one-third was strictly specific to plasminogen; the selectivity of this group was confirmed by immunoblots and BIA-core analysis. Cross-reactivity against horse, human, pig, sheep, rabbit, and bovine plasminogens was tested; 41% were strictly bovine specific.
通过用纯化的纤溶酶原免疫小鼠，制备了一组针对牛纤溶酶原的单克隆抗体。在一次融合实验（检测了1300个杂交瘤）中获得了300多个阳性克隆。通过它们在酶联免疫吸附测定（ELISA）中与纤溶酶和纤溶酶原的相对结合来鉴定四种类型的抗体。约三分之一的抗体对纤溶酶原具有严格的特异性；免疫印迹和生物传感器芯片分析证实了该组抗体的选择性。测试了对马、人、猪、羊、兔和牛纤溶酶原的交叉反应性；41%的抗体严格具有牛特异性。
