Ellis M Jane, Livesey John H, Soule Steven G
Endolab, Christchurch Hospital, Private Bag 4710, Christchurch, New Zealand.
Clin Biochem. 2006 Oct;39(10):1028-34. doi: 10.1016/j.clinbiochem.2006.06.003. Epub 2006 Jul 13.
To examine whether use of the Beckman Coulter Access Prolactin (PRL) assay, which has low reactivity with macro-PRL, obviates the need for screening hyperprolactinemic samples.
Samples from 1020 hyperprolactinemic individuals and 401 healthy volunteers were treated with polyethylene glycol (PEG). Macro-PRL was assessed from (1) percent PRL recovery, using cut-off values derived by gel filtration chromatography (GFC) and (2) significant (p<0.05) normalisation of PRL following PEG.
PRL recovery was similar in volunteer and hyperprolactinemic samples (mean+/-SD 101+/-13% and 101+/-19%, respectively). In hyperprolactinemic samples, macro-PRL was identified from PRL recovery in 9.7%, although levels were moderate to high in only 3.9%. The total PRL normalised following PEG in 7.4%. Correlations of PRL recovery with the proportions of macro-, big- and monomeric PRL following GFC (n=30 samples, range of PRL and macro-PRL levels) were -0.89, -0.20 and 0.92, respectively. The big-PRL content was 0-28%. Regression analysis suggested that PEG precipitated both macro-PRL and big-PRL.
Using the Access assay, macro-PRL can cause apparent hyperprolactinemia and big-PRL may cause misclassification of individuals. Screening using PEG is applicable to assays with low macro-PRL reactivity provided specific reference values are derived.
检测贝克曼库尔特Access催乳素（PRL）检测法（对大分子PRL反应性较低）是否无需筛查高催乳素血症样本。
对1020例高催乳素血症患者和401名健康志愿者的样本进行聚乙二醇（PEG）处理。通过以下方法评估大分子PRL：（1）使用凝胶过滤色谱法（GFC）得出的临界值计算PRL回收率百分比；（2）PEG处理后PRL显著（p<0.05）归一化。
志愿者样本和高催乳素血症样本的PRL回收率相似（分别为均值±标准差101±13%和101±19%）。在高催乳素血症样本中，9.7%的样本通过PRL回收率鉴定出大分子PRL，不过只有3.9%的样本水平为中度至高度。7.4%的样本经PEG处理后总PRL归一化。GFC后（n = 30个样本，PRL和大分子PRL水平范围）PRL回收率与大分子、大PRL和单体PRL比例的相关性分别为-0.89、-0.20和0.92。大PRL含量为0 - 28%。回归分析表明PEG沉淀了大分子PRL和大PRL。
使用Access检测法时，大分子PRL可导致明显的高催乳素血症，大PRL可能导致个体分类错误。只要得出特定参考值，使用PEG进行筛查适用于对大分子PRL反应性较低的检测法。
