Ellis M Jane, Reed Maxine R, Livesey John H
Endolab, 21 St Asaph St, Christchurch Hospital, Private Bag 4710, Christchurch, New Zealand.
Clin Biochem. 2007 Nov;40(16-17):1285-90. doi: 10.1016/j.clinbiochem.2007.08.002. Epub 2007 Aug 11.
To compare the recently released Elecsys Prolactin II with the Access and Centaur assays for reactivity with macroPRL, bigPRL and monomeric PRL in samples fractionated using gel filtration chromatography (GFC).
Prolactin (PRL) concentration was measured before (total PRL) and after GFC over Superdex 75 (n=16-18) using prolactin assays on the Access2 (Beckman Coulter Inc.) and Centaur (Siemens Medical Solutions Diagnostics) analyzers and the Prolactin II assay (Roche Diagnostics) on the Elecsys 2010 analyzer. The amounts of macroPRL, bigPRL and monomeric PRL were quantified from GFC peak areas.
Total PRL concentrations in macroPRL-containing specimens were (means+/-SD, n=13), 842+/-496; 851+/-564 mIU/L (Access and Elecsys II, p>0.05) and 695+/-469 mIU/L (Centaur, p<0.05). Monomeric PRL (GFC peak area) was lower by the Centaur (p<0.05; Deming regression) than by the Access or Elecsys II assays. Method comparisons (Bland and Altman) therefore used PRL peak areas expressed as percent total PRL recovered after GFC. The mean differences for macroPRL and bigPRL, respectively, were (a) Elecsys PRL II-Access assay: -2.83% and -2.54% (both p<0.05); (b) Elecsys PRL II-Centaur: -1.56% (p>0.05) and -6.48% (p<0.05) and (c) Access-Centaur: 1.41% (p>0.05) and -3.95% (p<0.05).
The new Elecsys Prolactin II assay has similar cross-reaction with macroPRL and bigPRL to the Access and Centaur Prolactin assays. The differences detected were small and unlikely to have clinical impact.
比较最近发布的罗氏电化学发光免疫分析法第二代催乳素检测试剂（Elecsys Prolactin II）与贝克曼库尔特公司的Access和西门子医疗解决方案诊断公司的Centaur检测方法，在使用凝胶过滤色谱法（GFC）分离的样本中对大分子催乳素（macroPRL）、大催乳素（bigPRL）和单体催乳素（monomeric PRL）的反应性。
使用贝克曼库尔特公司的Access2分析仪、西门子医疗解决方案诊断公司的Centaur分析仪以及罗氏诊断公司的Elecsys 2010分析仪上的催乳素检测方法，在Superdex 75上进行GFC分离之前（总催乳素）和之后（n = 16 - 18）测量催乳素（PRL）浓度。从GFC峰面积定量分析macroPRL、bigPRL和单体PRL的含量。
含macroPRL样本中的总PRL浓度（均值±标准差，n = 13）为，842±496；851±564 mIU/L（Access和Elecsys II，p>0.05）以及695±469 mIU/L（Centaur，p<0.05）。Centaur检测的单体PRL（GFC峰面积）低于Access或Elecsys II检测（p<0.05；Deming回归）。因此，方法比较（Bland和Altman）使用以GFC后回收的总PRL百分比表示的PRL峰面积。macroPRL和bigPRL的平均差异分别为：（a）Elecsys PRL II - Access检测：-2.83%和-2.54%（均p<0.05）；（b）Elecsys PRL II - Centaur检测：-1.56%（p>0.05）和-6.48%（p<0.05）；（c）Access - Centaur检测：1.41%（p>0.05）和-3.95%（p<0.05）。
新的Elecsys Prolactin II检测方法与macroPRL和bigPRL的交叉反应与Access和Centaur催乳素检测方法相似。检测到的差异很小，不太可能产生临床影响。
