Spielmann Tobias, Dixon Matthew W A, Hernandez-Valladares Maria, Hannemann Mandy, Trenholme Katharine R, Gardiner Donald L
Infectious Diseases and Immunology Division, Queensland Institute of Medical Research, PO Royal Brisbane Hospital, Qld 4029, Australia.
Int J Parasitol. 2006 Oct;36(12):1245-8. doi: 10.1016/j.ijpara.2006.06.017. Epub 2006 Jul 28.
Transfection of Plasmodium falciparum is instrumental in the research on this parasite. However, the actual transfection protocol has not changed significantly since the first description and it is generally believed that large amounts of highly pure plasmid DNA are needed for successful transfection. Here, we report the transfection of P. falciparum using a protocol based on non-commercial mini-preparations of plasmid DNA. This method permits the reliable transfection of P. falciparum using less resources and lower costs, with a success rate comparable with currently used methods. A moderate throughput may be achieved using this method, providing a first step towards systematic transfection approaches in this parasite.
恶性疟原虫的转染对于该寄生虫的研究至关重要。然而，自首次描述以来，实际的转染方案并没有显著改变，并且人们普遍认为成功转染需要大量高纯度的质粒DNA。在此，我们报告了使用基于非商业小规模制备质粒DNA的方案对恶性疟原虫进行转染。该方法能够以较少的资源和较低的成本可靠地转染恶性疟原虫，成功率与目前使用的方法相当。使用该方法可以实现适度的通量，为该寄生虫的系统转染方法迈出了第一步。
