Grüring Christof, Heiber Arlett, Kruse Florian, Ungefehr Johanna, Gilberger Tim-Wolf, Spielmann Tobias
Department of Molecular Parasitology, Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Street 74, Hamburg 20359, Germany.
Nat Commun. 2011 Jan 25;2:165. doi: 10.1038/ncomms1169.
Blood stages of Plasmodium falciparum cause the pathology of malaria; however, the progression of the parasite through this complex part of the life cycle has never been visualized. In this study, we use four-dimensional imaging to show for the first time the development of individual parasites in erythrocytes and the concomitant host cell modifications. Our data visualize an unexpectedly dynamic parasite, provide a reference for this life cycle stage and challenge the model that protein export in P. falciparum is linked to the biogenesis of host cell modifications termed Maurer's clefts. Our results provide a novel view of the blood-stage development, Maurer's cleft development and protein export in malaria parasites, and open the door to study dynamic processes, drug effects and the phenotype of mutants.
恶性疟原虫的血液阶段导致疟疾的病理变化；然而，寄生虫在这个复杂生命周期阶段的进展从未被可视化。在本研究中，我们首次使用四维成像来展示红细胞中单个寄生虫的发育以及伴随的宿主细胞变化。我们的数据可视化了一个出乎意料的动态寄生虫，为这个生命周期阶段提供了参考，并挑战了恶性疟原虫中蛋白质输出与称为毛雷尔氏小体的宿主细胞变化生物发生相关的模型。我们的结果为疟原虫的血液阶段发育、毛雷尔氏小体发育和蛋白质输出提供了新的视角，并为研究动态过程、药物作用和突变体表型打开了大门。
