Constantoulakis P, Nakamoto B, Papayannopoulou T, Stamatoyannopoulos G
Department of Medicine, University of Washington, Seattle 98195.
Blood. 1990 May 1;75(9):1862-9.
Cultures of peripheral blood or bone marrow erythroid progenitors display stimulated production of fetal hemoglobin. We investigated whether this stimulation is due to factors contained in the sera of the culture medium. Comparisons of gamma/gamma + beta biosynthetic ratios in erythroid colonies grown in fetal calf serum (FCS) or in charcoal treated FCS (C-FCS) showed that FCS-grown cells had significantly higher gamma/gamma + beta ratios. This increase in globin chain biosynthesis was reflected by an increase in relative amounts of steady-state gamma-globin mRNA. In contrast to its effect on adult cells, FCS failed to influence gamma-chain synthesis in fetal burst forming units-erythroid (BFU-E) colonies. There was a high correlation of gamma-globin expression in paired cultures done with C-FCS or fetal sheep serum. Dose-response experiments showed that the induction of gamma-globin expression is dependent on the concentration of FCS. These results indicate that FCS contains an activity that induces gamma-globin expression in adult erythroid progenitor cell cultures.
外周血或骨髓红系祖细胞培养显示胎儿血红蛋白生成受到刺激。我们研究了这种刺激是否归因于培养基血清中所含的因子。对在胎牛血清（FCS）或经活性炭处理的FCS（C-FCS）中生长的红系集落中γ/γ + β生物合成比率的比较表明，在FCS中生长的细胞具有显著更高的γ/γ + β比率。珠蛋白链生物合成的这种增加反映在稳态γ-珠蛋白mRNA相对量的增加上。与它对成年细胞的作用相反，FCS未能影响胎儿红系爆式形成单位（BFU-E）集落中的γ链合成。在用C-FCS或胎羊血清进行的配对培养中，γ-珠蛋白表达具有高度相关性。剂量反应实验表明，γ-珠蛋白表达的诱导取决于FCS的浓度。这些结果表明，FCS含有一种在成年红系祖细胞培养中诱导γ-珠蛋白表达的活性。
