Kawamura T, Shigesada K, Yanagisawa S, Izui K
Department of Chemistry, Faculty of Science, Kyoto University.
J Biochem. 1990 Jan;107(1):165-8. doi: 10.1093/oxfordjournals.jbchem.a123002.
In search of the gene family for phosphoenolpyruvate carboxylase (PEPC) [EC 4.1.1.31] in C4 plants, we isolated from a maize root cDNA library a new cDNA clone that cross-hybridized with a cDNA for PEPC involved in the C4 photosynthesis (Yanagisawa et al. (1988) FEBS Lett. 229, 107-110). Alignment of the nucleotide sequence with that of the probe cDNA revealed the absence of sequence homology in the 3' non-coding region between the two cDNAs. Southern blot hybridization probed with this specific sequence indicated that the corresponding gene is unique in the maize genome. Northern blot hybridization using the same probe showed that this gene-family member was expressed most strongly in roots and also in green leaves to a lesser extent, but not significantly in etiolated leaves.
为了在C4植物中寻找磷酸烯醇式丙酮酸羧化酶（PEPC）[EC 4.1.1.31]的基因家族，我们从玉米根cDNA文库中分离出一个新的cDNA克隆，它与参与C4光合作用的PEPC的cDNA发生了交叉杂交（柳泽等人（1988年）《欧洲生物化学学会联合会快报》229，107 - 110）。将该核苷酸序列与探针cDNA的序列进行比对，结果显示这两个cDNA在3'非编码区不存在序列同源性。用这个特定序列进行的Southern印迹杂交表明，相应的基因在玉米基因组中是唯一的。使用相同探针进行的Northern印迹杂交表明，该基因家族成员在根中表达最强，在绿叶中也有一定程度的表达，但在黄化叶中表达不明显。
