Albert H A, Martin T, Sun S S
Department of Plant Molecular Physiology, University of Hawaii, Honolulu 96822.
Plant Mol Biol. 1992 Nov;20(4):663-71. doi: 10.1007/BF00046451.
A gene (SCPEPCD1) encoding phosphoenolpyruvate carboxylase (PEPC) was isolated from the C-4 monocot sugarcane (Saccharum hybrid var. H32-8560). SCPEPCD1 is ca. 6800 bp long, with 10 exons. The entire gene sequence from -1561 to 262 bp downstream of the putative poly(A) addition signal is reported. A low-level, essentially constitutive pattern of expression, amino acid sequence similarities to other 'housekeeping' PEPC enzymes, and the absence of DNA sequence elements conserved in the upstream region of maize and sorghum C-4-specific PEPC genes indicate that SCPEPCD1 encodes a housekeeping PEPC. Despite this, a motif proposed to act as a phosphorylation site in light-mediated activation of photosynthetic PEPC enzymes [10] is present in the SCPEPCD1 protein; evidence is presented for the presence of this site in other housekeeping PEPC proteins.
从C4单子叶甘蔗（杂交种H32 - 8560）中分离出一个编码磷酸烯醇丙酮酸羧化酶（PEPC）的基因（SCPEPCD1）。SCPEPCD1约6800 bp长，有10个外显子。报道了从假定的多聚腺苷酸化信号下游 - 1561至262 bp的整个基因序列。其低水平、基本组成型的表达模式，与其他“管家”PEPC酶的氨基酸序列相似性，以及在玉米和高粱C4特异性PEPC基因上游区域不存在保守的DNA序列元件，表明SCPEPCD1编码一种管家PEPC。尽管如此，在SCPEPCD1蛋白中存在一个被认为在光合PEPC酶光介导激活中起磷酸化位点作用的基序[10]；文中提供了其他管家PEPC蛋白中存在该位点的证据。
