Polfliet Machteld M J, Fabriek Babs O, Daniëls Wouter P, Dijkstra Christine D, van den Berg Timo K
Department of Molecular Cell Biology and Immunology, VU Medical Center, 1007 MB Amsterdam, The Netherlands.
Immunobiology. 2006;211(6-8):419-25. doi: 10.1016/j.imbio.2006.05.015. Epub 2006 Jul 24.
The monoclonal antibody ED2 is widely used to define macrophages (mphi) in the rat. We have recently identified the ED2 antigen as the rat CD163 glycoprotein. CD163 is a member of the scavenger receptor cysteine-rich group B (SRCR-B) family and functions as a scavenger receptor for hemoglobin-haptoglobin complexes. Moreover, CD163 has also been indicated as a marker for alternatively activated mphi. In the current study, we identify rat CD163/ED2-antigen as a marker for mature tissue mphi. Rat CD163 is constitutively expressed on most subpopulations of mature tissue mphi, including splenic red pulp mphi, thymic cortical mphi, Kupffer cells in the liver, resident bone marrow mphi and central nervous system perivascular and meningeal mphi, but is apparently absent from monocytes. Rat CD163 expression can be promoted by glucocorticoids, and this can be further enhanced by IL4. Finally, engagement of rat CD163 on peritoneal mphi induces the production of pro-inflammatory mediators, including NO, IL-1beta, IL-6 and TNF-alpha. Collectively, our findings identify rat CD163 as a broadly expressed macrophage scavenger receptor that may play a role in the activation of mphi during hemolytic and/or inflammatory conditions.
单克隆抗体ED2被广泛用于鉴定大鼠体内的巨噬细胞（mphi）。我们最近已确定ED2抗原为大鼠CD163糖蛋白。CD163是富含半胱氨酸的清道夫受体B族（SRCR-B）家族的成员，作为血红蛋白-触珠蛋白复合物的清道夫受体发挥作用。此外，CD163也被认为是替代性活化mphi的标志物。在本研究中，我们确定大鼠CD163/ED2抗原为成熟组织mphi的标志物。大鼠CD163在成熟组织mphi的大多数亚群中组成性表达，包括脾红髓mphi、胸腺皮质mphi、肝库普弗细胞、驻留骨髓mphi以及中枢神经系统血管周围和脑膜mphi，但在单核细胞中明显不存在。大鼠CD163的表达可被糖皮质激素促进，而IL4可进一步增强这种促进作用。最后，腹膜mphi上大鼠CD163的结合可诱导促炎介质的产生，包括NO、IL-1β、IL-6和TNF-α。总的来说，我们的研究结果确定大鼠CD163为一种广泛表达的巨噬细胞清道夫受体，其可能在溶血和/或炎症状态下mphi的激活中发挥作用。
