Mothersill C, Seymour C B, Cusack A, O'Brien A, Butler M
Saint Luke's Hospital, Dublin Institute of Technology, Ireland.
Acta Oncol. 1990;29(2):179-84. doi: 10.3109/02841869009126542.
Using an explant tissue culture model developed by this group for use with human surgical and biopsy specimens, data are presented showing the response of normal and tumor bladder urothelium to radiation in combination with cis- and carboplatin. Cellular response is measured after two weeks in culture as a reduction in the extent of outgrowth from the explant. The outgrowth has been shown to be growing and to be epithelial. Results showed that when either drug or radiation is used singly, the tumour is resistant to treatment while the normal cells are severely affected. However, appropriate combinations of either drug with radiation reverse the unfavourable therapeutic ratio and result in higher tumour cell kill. The model may be useful for investigating mechanisms of radiation/chemotherapy action at the cellular level and, if integrated into appropriate clinical trials, may serve as an easy-to-use in vitro test for optimising single agent or combination therapy regimens.
利用该研究小组开发的用于人类手术和活检标本的外植体组织培养模型，本文呈现的数据表明了正常和肿瘤膀胱尿路上皮对辐射联合顺铂和卡铂的反应。培养两周后，通过测量外植体生长范围的减少来衡量细胞反应。已证明该生长物在生长且为上皮性的。结果显示，当单独使用药物或辐射时，肿瘤对治疗具有抗性，而正常细胞受到严重影响。然而，药物与辐射的适当组合可逆转不利的治疗比率，并导致更高的肿瘤细胞杀伤率。该模型可能有助于在细胞水平上研究放疗/化疗作用机制，并且如果整合到适当的临床试验中，可作为一种易于使用的体外试验，用于优化单药或联合治疗方案。
