肌钙蛋白T特异性钙调蛋白抗体池与原肌球蛋白对骨骼肌肌钙蛋白T和平滑肌钙调蛋白的竞争性结合。

Competitive binding of the troponin T-specific pool of caldesmon antibodies and tropomyosin to skeletal troponin T and smooth muscle caldesmon.

作者信息

Birukov K G, Shirinsky V P, Vorotnikov A V, Gusev N B

机构信息

Institute of Experimental Cardiology, USSR Cardiology Research Center, USSR Academy of Medical Sciences, Moscow.

出版信息

FEBS Lett. 1990 Mar 26;262(2):263-5. doi: 10.1016/0014-5793(90)80206-x.

DOI:10.1016/0014-5793(90)80206-x

PMID:1692290

Abstract

The fraction of polyclonal caldesmon antibodies cross-reacting with rabbit skeletal troponin T are shown to compete with smooth muscle tropomyosin for caldesmon and troponin T, as revealed by ELISA method. The epitope recognized by these antibodies was also found in Mr 77 kDa non-muscle caldesmon. These results provide functional confirmation for the suggestion that the regions of amino acid sequence homology in caldesmon isoforms and troponin T belong to the tropomyosin binding sites.

摘要

通过ELISA方法显示，与兔骨骼肌肌钙蛋白T发生交叉反应的多克隆钙调蛋白抗体部分，可与平滑肌原肌球蛋白竞争结合钙调蛋白和肌钙蛋白T。这些抗体识别的表位也存在于77 kDa的非肌肉钙调蛋白中。这些结果为如下观点提供了功能上的证实，即钙调蛋白异构体和肌钙蛋白T中氨基酸序列同源的区域属于原肌球蛋白结合位点。