Jin Shin Dong, Gan-Undram Semeibsuren, Jin Kim Seo, Joon Jun Young, Jung Im Gi, Hyun Jung Hak
Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul, Republic of Korea.
Acta Otolaryngol. 2006 Oct;126(10):1040-5. doi: 10.1080/00016480600672626.
Since the expression levels of beta-defensins 2-4 were up-regulated in experimental otitis media, they may play a protective role in the pathogenesis of otitis media.
Defensins are antimicrobial peptides that play a major role in innate immunity. The goal of this study was to identify the expression of defensins in experimental otitis media of the mouse.
The expression of three alpha-defensins (cryptdins, cryptdin-related sequences 1-C (CRS1-C), and CRS4-C) and four beta-defensins (mBD1, mBD2, mBD3, and mBD4) was investigated in the tubotympanum of experimental otitis media in mice by a semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), and the expression levels of three beta-defensins (mBD2, mBD3, mBD4) were evaluated by Western blotting.
The alpha-defensins were not expressed in the tubotympanum. mBD1 was expressed constitutively in normal middle ear mucosa and Eustachian tube mucosa, but up-regulated expression of mBD2, mBD3, and mBD4 was observed with RT-PCR and Western blotting in the tubotympanums in experimental otitis media, while the normal tubotympanums did not express them.
由于β-防御素2 - 4在实验性中耳炎中表达水平上调，它们可能在中耳炎发病机制中发挥保护作用。
防御素是在固有免疫中起主要作用的抗菌肽。本研究的目的是确定防御素在小鼠实验性中耳炎中的表达情况。
通过半定量逆转录 - 聚合酶链反应（RT-PCR）研究三种α-防御素（隐窝素、隐窝素相关序列1 - C（CRS1 - C）和CRS4 - C）以及四种β-防御素（mBD1、mBD2、mBD3和mBD4）在小鼠实验性中耳炎鼓室咽鼓管中的表达，并通过蛋白质印迹法评估三种β-防御素（mBD2、mBD3、mBD4）的表达水平。
α-防御素在鼓室咽鼓管中未表达。mBD1在正常中耳黏膜和咽鼓管黏膜中组成性表达，但在实验性中耳炎的鼓室咽鼓管中，通过RT-PCR和蛋白质印迹法观察到mBD2、mBD3和mBD4表达上调，而正常鼓室咽鼓管不表达它们。
